53 Result(s)
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Article
Minghong Gu
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Article
Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) Moench
Immature embryos of 20 sorghum genotypes were cultured on MS 5 medium containing MS mineral salts supplemented with 2,4-D, zeatin, glycine, niacinamide, Ca-pantothenate, L-asparagine, and vitamins. For regener...
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Article
Microdissection and amplification of the chromosome arm 5S in a rice telo-tetrasomic
The rice aneuploids with telochromosomes are ideal genetic stocks for chromosome arm identification and microdissection. A rice telo-tetrasomic line with two extra short arms of chromosome 5 (5S) was used in t...
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A tandemly repeated DNA sequence is associated with both knob-like heterochromatin and a highly decondensed structure in the meiotic pachytene chromosomes of rice
Highly repetitive tandem DNA sequence repeats are often associated with centromeric and telomeric regions of eukaryotic chromosomes. The rice tandem repeat Os48 is organized as long arrays of a 355 bp monomer...
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Introduction of antisensewaxy gene into main parent lines ofindica hybrid rice
Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality ofindica hybrid rice is closely related to the high amylose level in rice grains. In order...
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Genetic map** of a new semi-dwarf gene, sd-t(t), in indica rice and estimating of the physical distance of the map** region
Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1, had been identified in an i...
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Sequence and analysis of rice chromosome 4
Rice is the principal food for over half of the population of the world. With its genome size of 430 megabase pairs (Mb), the cultivated rice species Oryza sativa is a model plant for genome research1. Here we re...
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Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality
Amylose content in rice endosperm is a key determinant of eating and cooking quality. In the present study, a chimeric antisense construct, which contained a 756-bp antisense Waxy (Wx) gene DNA fragment from rice...
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Develo** gene-tagged molecular markers for functional analysis of starch-synthesizing genes in rice (Oryza sativa L.)
The granule-bound starch synthase(GBSS), starch branching enzymes 1 (SBE1)and 3 (SBE3) are major enzymes involved in starch biosynthesis in rice endosperm. Available sequences of Sbe1 and Sbe3 genes encoding corr...
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Fine map** of a semidwarf genesd-g in indica rice (Oryza sativa L.)
The semidwarf genesd-g which has been used in indica rice breeding in southern China is a new one, nonallelic tosd-1. To mapsd-g, an F2 population derived from the cross between **nguiaishuangai and 02428 was con...
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Article
Isolation and identification of the three rice monotelosomics
In order to obtain rice monotelosomic, the progeny of 24 telotrisomics, derived from anindica rice variety, Zhongxian 3037, were screened. The variants that differed morphologically from the diploids and the orig...
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Article
Delimiting a rice wide-compatibility gene S 5 n to a 50 kb region
Wide-compatibility (WC) is one of the most important traits in rice, which can overcome the fertility barrier in the indica/japonica hybrids, and hence to make it possible to utilize the higher yield potential of...
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Article
Genetic analysis and gene fine map** for a rice novel mutant (rl 9(t) ) with rolling leaf character
Leaf shape is an important parameter for ideotype breeding in rice, and the rolling of leaf is also beneficial to efficient ripening of grains. This encourages the explorations of new genes that regulate leaf ...
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Diversity of centromeric repeats in two closely related wild rice species, Oryza officinalis and Oryza rhizomatis
Oryza officinalis (CC, 2n=24) and Oryza rhizomatis (CC, 2n=24) belong to the Oryza genus, which contains more than 20 identified wild rice species. Although much has been known about the ...
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Molecular map** of two semidwarf genes in an indica rice variety Aitaiyin3 (Oryza sativa L.)
Genetic analysis established that Aitaiyin3, a dwarf rice variety derived from a semidwarf cultivar Taiyin1, carries two recessive semidwarf genes. By using simple sequence repeat (SSR) markers, we mapped the ...
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Genetic analysis and molecular map** of a presenescing leaf gene psl1 in rice (Oryza sativa L.)
A rice psl1 (presenescing leaf) mutant was obtained from a japonica variety Zhonghua 11 via radiation of 60Co-y in M2 generation. Every leaf of the mutant began to wither after it reached the biggest length, whil...
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Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization
Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits...
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Generating of rice OsCENH3-GFP transgenic plants and their genetic applications
In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exoge...
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Article
Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation
During sexual propagation of primary trisomic 8, chromosome 8 breaks in some rice plants, resulting in a telotrisomic (2n+·8S) line. In this study, we observed that the extra short arm of chromosome 8 (·8S) ca...
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Genetic analysis and gene fine map** of a rolling leaf mutant (rl 11(t) ) in rice (Oryza sativa L.)
The exploration of new genes controlling rice leaf shape is an important foundation for rice functional genomics and plant architecture improvement. In the present study, we identified a rolling leaf mutant from
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Development of gene-tagged molecular markers for starch synthesis-related genes in rice
Improving grain quality is an important goal in breeding new elite rice varieties, requiring effective tools for the identification of target genotypes. Molecular marker-aided selection (MAS), combined with co...
