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Article
Plant regeneration from cultured immature embryos of Sorghum bicolor (L.) Moench
Immature embryos of 20 sorghum genotypes were cultured on MS 5 medium containing MS mineral salts supplemented with 2,4-D, zeatin, glycine, niacinamide, Ca-pantothenate, L-asparagine, and vitamins. For regener...
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Article
Stable Inheritance of the Antisense Waxy Gene in Transgenic Rice with Reduced Amylose Level and Improved Quality
Amylose content in rice endosperm is a key determinant of eating and cooking quality. In the present study, a chimeric antisense construct, which contained a 756-bp antisense Waxy (Wx) gene DNA fragment from rice...
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Article
Develo** gene-tagged molecular markers for functional analysis of starch-synthesizing genes in rice (Oryza sativa L.)
The granule-bound starch synthase(GBSS), starch branching enzymes 1 (SBE1)and 3 (SBE3) are major enzymes involved in starch biosynthesis in rice endosperm. Available sequences of Sbe1 and Sbe3 genes encoding corr...
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Article
Delimiting a rice wide-compatibility gene S 5 n to a 50 kb region
Wide-compatibility (WC) is one of the most important traits in rice, which can overcome the fertility barrier in the indica/japonica hybrids, and hence to make it possible to utilize the higher yield potential of...
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Article
Diversity of centromeric repeats in two closely related wild rice species, Oryza officinalis and Oryza rhizomatis
Oryza officinalis (CC, 2n=24) and Oryza rhizomatis (CC, 2n=24) belong to the Oryza genus, which contains more than 20 identified wild rice species. Although much has been known about the ...
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Article
Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation
During sexual propagation of primary trisomic 8, chromosome 8 breaks in some rice plants, resulting in a telotrisomic (2n+·8S) line. In this study, we observed that the extra short arm of chromosome 8 (·8S) ca...
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Article
Open AccessDevelo** high throughput genotyped chromosome segment substitution lines based on population whole-genome re-sequencing in rice (Oryza sativa L.)
Genetic populations provide the basis for a wide range of genetic and genomic studies and have been widely used in genetic map**, gene discovery and genomics-assisted breeding. Chromosome segment substitutio...
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Article
Fine map** of a major QTL controlling panicle number in rice
Rice panicle number per plant is a grain yield component that directly influences rice yield. The identification of the genes controlling panicle number will play a vital role in high-yield rice breeding. C307...
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Article
Fine map** and candidate gene analysis of ptgms2-1, the photoperiod-thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)
Photoperiod-thermo-sensitive genic male sterile (PTGMS) rice exhibits a number of desirable traits for hybrid rice production. The cloning genes responsible for PTGMS and those elucidating male sterility mecha...
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Article
Hd-q, a novel allele of Ef7 from a Chinese rice landrace, confers weak photoperiod sensitivity and improves local adaptability and yield potential
Heading date is a major determinant of adaptability and yield potential in rice (Oryza sativa L.) and is influenced by photoperiod. Among chromosome segment substitution lines, the introgression line C63 contains...
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Article
Identification and diversity of functional centromere satellites in the wild rice species Oryza brachyantha
The centromere is a key chromosomal component for sister chromatid cohesion and is the site for kinetochore assembly and spindle fiber attachment, allowing each sister chromatid to faithfully segregate to each...
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Article
Identification and fine map** of qPH6, a novel major quantitative trait locus for plant height in rice
Plant height is one of the determinants that influence rice plant architecture and grain yield. Identification of useful novel genes related to plant height is necessary for breeding high-yield rice. In this s...
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Article
Identification of quantitative trait loci responsible for rice grain protein content using chromosome segment substitution lines and fine map** of qPC-1 in rice (Oryza sativa L.)
Protein content (PC) in rice endosperm plays an important role in determining rice grain quality. However, the genetic mechanism underlying grain PC remains unclear. In order to better understand the genetic b...
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Article
Genetic dissection of hybrid breakdown in an indica/japonica cross and fine map** of a quantitative trait locus qSF-12 in rice (Oryza sativa L.)
Hybrid breakdown is an important form of post-zygotic reproductive barriers, often arising from hybrid progeny between two varietal groups of Asian cultivated rice (Oryza sativa L.), indica and japonica. However,...
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Article
Rf5 is able to partially restore fertility to Honglian-type cytoplasmic male sterile japonica rice (Oryza sativa) lines
Three-line japonica hybrids have been developed mainly on Chinsurah Boro II (BT)-type cytoplasmic male sterile (CMS) lines of Oryza sativa L., but the unstable sterility of some BT-type C...
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Article
Map** QTLs conferring resistance to rice black-streaked dwarf disease in rice (Oryza sativa L)
Rice black-streaked dwarf disease, caused by the rice black-streaked dwarf virus (RBSDV), can lead to severe yield losses in rice. The deployment of resistant cultivars is an effective disease control measure,...
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Article
Open AccessAbility of Rf5 and Rf6 to Restore Fertility of Chinsurah Boro II-type Cytoplasmic Male Sterile Oryza Sativa (ssp. Japonica) Lines
Three-line Oryza sativa (ssp. japonica) hybrids have been developed mainly using Chinsurah Boro II (BT)-type cytoplasmic male sterility (CMS). The Rf1 gene restores the fertility of BT-type CMS lines, and is the ...
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Article
RNAi-mediated resistance to rice black-streaked dwarf virus in transgenic rice
Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus in the family Reoviridae, causes significant economic losses in rice production in China and many other Asian countries. Development of res...
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Article
Rf4 has minor effects on the fertility restoration of wild abortive-type cytoplasmic male sterile japonica (Oryza sativa) lines
Wild abortive (WA)-type cytoplasmic male sterility (CMS) has been exclusively used for breeding three-line hybrid indica rice, but it has not been applied for generating japonica hybrids because of the difficulti...
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Article
Open AccessEvaluation of differential qPE9-1/DEP1 protein domains in rice grain length and weight variation
qPE9–1/DEP1, encoding a G protein γ subunit, has multiple effects on plant architecture, grain size, and yield in rice. The qPE9–1 protein contains an N-terminal G gamma-like (GGL) domain, a putative transmembran...