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    Article

    Methoxyflavones isolated from the whole plant of Scutellaria rubropunctata Hayata var. rubropunctata promote osteoblast differentiation in MC3T3-E1 cells

    In this study, we isolated two new methoxyflavones (1 and 2) and eight known methoxyflavones (3–10) from the whole plant of Scutellaria rubropunctata Hayata var. rubropunctata (SR). Based on spectroscopic analyse...

    Misaki Watanabe, Tadahiro Yahagi, Rina Kamikura… in Journal of Natural Medicines (2023)

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    Article

    T-cell responses and combined immunotherapy against human carbonic anhydrase 9-expressing mouse renal cell carcinoma

    Renal cell carcinoma (RCC) is known to respond to immune checkpoint blockade (ICB) therapy, whereas there has been limited analysis of T-cell responses to RCC. In this study, we utilized human carbonic anhydra...

    Mamoru Harada, Yuichi Iida, Hitoshi Kotani… in Cancer Immunology, Immunotherapy (2022)

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    Article

    Elimination of mycoplasmas from cell cultures by a novel soft agar technique

    Mycoplasmal infection of cell cultures remains a significant threat to diagnostic and research procedures. In certain defined situations, curing of mycoplasmal infected cultures is a reasonable exercise. Four ...

    Hitoshi Kotani, Gary Butler, Diane Heggan… in In Vitro Cellular & Developmental Biology … (1991)

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    Article

    Microbiological cultivation ofMycoplasma hyorhinis from cell cultures

    The failure of many cell culture isolates ofMycoplasma hyorhinis to grow on microbiological media has stressed the need for alternate assays to detect these organisms. The use of freshly prepared yeast extract in...

    Hitoshi Kotani, Gary H. Butler… in In Vitro Cellular & Developmental Biology (1990)

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    Article

    Comparative studies to determine the efficiency of 6 methylpurine deoxyriboside to detect cell culture mycoplasmas

    Studies were performed to compare three methods to detect mycoplasmal infection of cell cultures. The methods included microbiological assay by inoculation into broth and onto agar with anaerobic incubation, f...

    Gerard J. McGarrity, Hitoshi Kotani… in In Vitro Cellular & Developmental Biology (1986)

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    Article

    Uridine phosphorylase activity among the class mollicutes

    Uridine phosphorylase activity has been used to detect mycoplasmas in cell cultures by measuring formation of14C-uracil from14C-uridine. In this report we show that all species ofMycoplasma, Acholeplasma, andUrea...

    Dr. Gerard J. McGarrity, Lindsay Gamon, Theodor Steiner… in Current Microbiology (1985)