Editor’s statement This manuscript describes procedures for the cultivation of mycoplasma strains that previously were not cultivatable.
Summary
The failure of many cell culture isolates ofMycoplasma hyorhinis to grow on microbiological media has stressed the need for alternate assays to detect these organisms. The use of freshly prepared yeast extract in mycoplasmal media together with incubation in 5% CO2/air successfully detectedM. hyorhinis in 12 of 12 infected cultures. These were not detected by the use of conventional mycoplasmal media using aerobic or anaerobic incubation. This assay may also be helpful in detection of other mycoplasmal species commonly isolated from cell cultures.
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This study was supported by grant AI 15748 from NIH. We thank Ronnie Vanaman and Judi Sarama for their technical assistance.
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Kotani, H., Butler, G.H., Tallarida, D. et al. Microbiological cultivation ofMycoplasma hyorhinis from cell cultures. In Vitro Cell Dev Biol 26, 91–96 (1990). https://doi.org/10.1007/BF02624161
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DOI: https://doi.org/10.1007/BF02624161