30 Result(s)
within
Article
Annemarie H. Meijer
-
Article
Open AccessMycobacterium tuberculosis β-lactamase variant reduces sensitivity to ampicillin/avibactam in a zebrafish-Mycobacterium marinum model of tuberculosis
The β-lactamase of Mycobacterium tuberculosis, BlaC, hydrolyzes β-lactam antibiotics, hindering the use of these antibiotics for the treatment of tuberculosis. Inhibitors, such as avibactam, can reversibly inhibi...
-
Article
Open AccessThe adapter protein Myd88 plays an important role in limiting mycobacterial growth in a zebrafish model for tuberculosis
Tuberculosis (TB) is the most prevalent bacterial infectious disease in the world, caused by the pathogen Mycobacterium tuberculosis (Mtb). In this study, we have used Mycobacterium marinum (Mm) infection in zebr...
-
Article
Open AccessDeficiency in the autophagy modulator Dram1 exacerbates pyroptotic cell death of Mycobacteria-infected macrophages
DNA damage regulated autophagy modulator 1 (DRAM1) is a stress-inducible regulator of autophagy and cell death. DRAM1 has been implicated in cancer, myocardial infarction, and infectious diseases, but the mole...
-
Article
Open AccessInfection and RNA-seq analysis of a zebrafish tlr2 mutant shows a broad function of this toll-like receptor in transcriptional and metabolic control and defense to Mycobacterium marinum infection
The function of Toll-like receptor 2 (TLR2) in host defense against pathogens, especially Mycobacterium tuberculosis (Mtb) is poorly understood. To investigate the role of TLR2 during mycobacterial infection, we ...
-
Article
Open AccessAspergillus fumigatus establishes infection in zebrafish by germination of phagocytized conidia, while Aspergillus niger relies on extracellular germination
Among opportunistically pathogenic filamentous fungi of the Aspergillus genus, Aspergillus fumigatus stands out as a drastically more prevalent cause of infection than others. Utilizing the zebrafish embryo model...
-
Article
Adverse outcome pathways: opportunities, limitations and open questions
Adverse outcome pathways (AOPs) are a recent toxicological construct that connects, in a formalized, transparent and quality-controlled way, mechanistic information to apical endpoints for regulatory purposes....
-
Article
Open AccessExpression and regulation of drug transporters in vertebrate neutrophils
There remains a need to identify novel pro-resolution drugs for treatment of inflammatory disease. To date, there are no neutrophil-specific anti-inflammatory treatments in clinical use, perhaps due to our lac...
-
Article
Open AccessThe chemokine receptor CXCR4 promotes granuloma formation by sustaining a mycobacteria-induced angiogenesis programme
CXC chemokine receptor 4 plays a critical role in chemotaxis and leukocyte differentiation. Furthermore, there is increasing evidence that links this receptor to angiogenesis. Using the well-established zebraf...
-
Article
Open AccessProtection and pathology in TB: learning from the zebrafish model
Zebrafish has earned its place among animal models of tuberculosis. Its natural pathogen, Mycobacterium marinum, shares major virulence factors with the human pathogen Mycobacterium tuberculosis. In adult zebrafi...
-
Article
Open AccessCommon and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish
Although the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole or...
-
Article
Open AccessAnalysis of RNAseq datasets from a comparative infectious disease zebrafish model using GeneTiles bioinformatics
We present a RNA deep sequencing (RNAseq) analysis of a comparison of the transcriptome responses to infection of zebrafish larvae with Staphylococcus epidermidis and Mycobacterium marinum bacteria. We show how o...
-
Article
Open AccessParallel deep transcriptome and proteome analysis of zebrafish larvae
Sensitivity and throughput of transcriptomic and proteomic technologies have advanced tremendously in recent years. With the use of deep sequencing of RNA samples (RNA-seq) and mass spectrometry technology for...
-
Article
Open AccessMicroRNA-146 function in the innate immune transcriptome response of zebrafish embryos to Salmonella typhimurium infection
MicroRNAs (miRNAs) have recently been shown to play important roles in development of the immune system and in fine-tuning of immune responses. Human miR-146 family members are known as inflammation-inducible ...
-
Article
Open AccessA zebrafish high throughput screening system used for Staphylococcus epidermidisinfection marker discovery
Staphylococcus epidermidis bacteria are a major cause of biomaterial-associated infections in modern medicine. Yet there is little known about the host responses against this normally innocent bacterium in the co...
-
Article
Function of the HD-Zip I gene Oshox22 in ABA-mediated drought and salt tolerances in rice
Oshox22 belongs to the homeodomain-leucine zipper (HD-Zip) family I of transcription factors, most of which have unknown functions. Here we show that the expression of Oshox22 is strongly induced by salt stress, ...
-
Article
Open AccessComparison of static immersion and intravenous injection systems for exposure of zebrafish embryos to the natural pathogen Edwardsiella tarda
The zebrafish embryo is an important in vivo model to study the host innate immune response towards microbial infection. In most zebrafish infectious disease models, infection is achieved by micro-injection of ba...
-
Article
Open AccessRapid screening of innate immune gene expression in zebrafish using reverse transcription - multiplex ligation-dependent probe amplification
With the zebrafish increasingly being used in immunology and infectious disease research, there is a need for efficient molecular tools to evaluate immune gene expression in this model species. RT-MLPA (revers...
-
Article
Open AccessZebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine
Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in ...
-
Article
A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most ab...
-
Article
A Homeodomain Leucine Zipper Gene from Craterostigma plantagineum Regulates Abscisic Acid Responsive Gene Expression and Physiological Responses
A subset of homeodomain leucine zipper proteins (HDZip) play a role in regulating adaptation responses including developmental adjustment to environmental cues in plants. Here we report the structural and func...
