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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches
Diseases caused by Capripoxviruses (CaPVs) are of great economic importance in sheep, goats, and cattle. Since CaPV strains are serologically... -
Freshwater Viral Metagenome Analyses Targeting dsDNA Viruses
Viral metagenomics is one of the most widely used approaches to study viral population genomics. With the recent development of bioinformatic tools,... -
Cancer Sample Analysis Utilizing Single-Nucleotide Polymorphism Array and Array Comparative Genomic Hybridization
Chromosomal microarray, including single-nucleotide polymorphism (SNP) array and array comparative genomic hybridization (aCGH), enables the... -
Profiling Chromosome Topological Features by Super-Resolution 3D Structured Illumination Microscopy
Three-dimensional structured illumination microscopy (3D-SIM) and fluorescence in situ hybridization on three-dimensional preserved cells (3D-FISH)... -
Methods of Detection and Mechanisms of Origin of Complex Structural Genome Variations
Based on classical karyoty**, structural genome variations (SVs) have generally been considered to be either “simple” (with one or two breakpoints)... -
Evaluating Chromosome Instability and Genotoxicity Through Single Cell Quantitative Imaging Microscopy
Across eukaryotes, genome stability is essential for normal cell function, physiology, and species survival. Aberrant expression of key genes or... -
Studying the Dynamics of Tunneling Tubes and Cellular Spheres
Cancer cytogenetic analyses often involve cell culture. However, many cytogeneticists overlook interesting phenotypes associated with cultured cells.... -
Characterizing Chemotherapy/Radiotherapy-Induced Genome Chaos in Hodgkin’s Lymphoma Patients Using M-FISH
Hodgkin lymphoma (HL) is one of the most common lymphomas, with an incidence of 3 per 100,000 persons. Current treatment uses a cocktail of genotoxic... -
The Digital World of Cytogenetic and Cytogenomic Web Resources
The dynamic growth of technological capabilities at the cellular and molecular level has led to a rapid increase in the amount of data on the genes... -
High-Resolution FISH Analysis Using DNA Fibers Generated by Molecular Combing
Molecular combing is a technique used to stretch hundreds of consistent DNA molecules in parallel on a glass surface, with a resolution of two... -
Enrichment, Sequencing, and Identification of DNA Bacteriophages from Fecal Samples
Research on individual viruses and phages, as well as viral populations (viromes), is greatly expanding. Phages and viromes are increasingly... -
Viromes of Plants Determined by High-Throughput Sequencing of Virus-Derived siRNAs
Plants growing in open airfields can be infected by several viruses even as a multiple infection. Virus infection in crops can lead to a serious... -
Metagenomic Analyses of Viruses in the Orchid Mycorrhizal Interaction Using Improved Assemble Tools
In nature, mycorrhizal association with soil-borne fungi is indispensable for orchid species. Compatible mycorrhizal fungi form endo-mycorrhizal... -
From Metagenomics to Ecogenomics: NGS-Based Approaches for Discovery of New Circular DNA Single-Stranded Viral Species
Viruses comprise the most abundant genetic material in the biosphere; however, global viral genomic population (virome) has been largely... -
Identification of DNA Viruses in Ancient DNA from Herbarium Samples
Herbaria encompass millions of plant specimens, mostly collected in the nineteenth and twentieth centuries that can represent a key resource for... -
Application of Nanopore-Based Sequencing to Identify Virus Infections in Woody Plants
Plant viruses threaten the yield and quality of crops. Efficient and affordable pathogen diagnosis is crucial to regulate the trade of plant... -
Recovery of Recombinant Rotaviruses by Reverse Genetics
Rotaviruses are the primary cause of severe gastroenteritis in infants and young children throughout the world. To combat rotavirus illness, several... -
Plasmid-Based Lassa Virus Reverse Genetics
Several mammarenaviruses cause hemorrhagic fever (HF) disease in humans and pose a significant public health problem in their endemic regions. The... -
Reverse Genetics of Hepatitis C Virus Using an RNA Polymerase I-Mediated Transcription
The reverse genetics system commonly used for the production of hepatitis C virus (HCV), which is a major causative agent of liver diseases, involves... -
Exploring the Archaeal Virosphere by Metagenomics
During the past decade, environmental research has demonstrated that archaea are abundant and widespread in nature and play important ecological...