3,937 Result(s)
within
English
2012 - 2012
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Protocol
Skin Sensitivity Studies
Sensitive skin is defined as the occurrence of erythema and/or abnormal stinging, burning, and tingling sensations (and sometimes as pain or pruritus) in response to multiple factors that may be physical (UV, ...
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Balloon Injury in Rats as a Model for Studying TRP Channel Contribution to Vascular Smooth Muscle Remodeling
Many vascular occlusive diseases are characterized by endothelial dysfunction and phenotypic switch of vascular smooth muscle cell (VSMC) from quiescent contractile to proliferative migratory phenotypes. These...
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Chick Anxiety–Depression Screening Model
The chick anxiety–depression model is a hybrid, dual pharmacological screening assay in which both anxiety and depression present sequentially over a 2 h isolation period. This separation stress paradigm utili...
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Use of Cell-Stretch System to Examine the Characteristics of Mechanosensor Channels: Axonal Growth/Neuroregeneration Studies
Temperature-sensitive TRP (so-called “thermoTRP”) channels are well recognized for their contributions to sensory transduction, responding to a wide variety of stimuli including temperature, nociceptive stimul...
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Morphological Assessments of Neonatal Hypoxia–Ischemia: White Matter and Blood-Brain Barrier Injury
Damages to the blood–brain barrier (BBB) and white matter (WM)/oligodendrocytes (OLs) are typical pathological findings in infants or animal models of neonatal hypoxia–ischemia (NHI). These injuries in turn pr...
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Assessment of Microthromboembolism After Subarachnoid Hemorrhage
Delayed cerebral ischemia is a common complication after aneurysmal subarachnoid hemorrhage (SAH). Besides arterial narrowing (vasospasm), microthromboembolism has been suggested as an additional explanation f...
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Assessment of Angiogenesis in Models of Focal Cerebral Ischemia
Angiogenesis could be interpreted as a natural defense mechanism, hel** to restore oxygen and nutrient supplies to the affected brain tissue. It has been demonstrated that angiogenesis is involved in functio...
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Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells
Conventionally, human embryonic stem cells (hESCs) are cultured on feeder cells. The most commonly used feeder cells are mouse embryonic fibroblasts. It is thought that the feeder cells provide an optimal micr...
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Morphological Assessments of Global Cerebral Ischemia: Degenerated Cells
Global cerebral ischemia (GCI) results in neurodegeneration in a variety of brain regions. Accumulated evidences suggest that DNA damages are involved in apoptotic cell death in cases of GCI. Klenow labeling a...
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In Vitro Two-Dimensional Endothelial Differentiation of Human Embryonic Stem Cells
Pluripotent human embryonic stem cells (hESCs) comprise of cells from all three germ layers in vivo. They have been proved to differentiate in vitro into a variety of cell lineages. Endothelial cells derived f...
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Morphological Assessments of Cerebral Vasospasm
Morphological changes of vasospastic arteries have already been investigated in many studies, and its details have been clarified. However, morphological assessments are still fundamental and essential in rese...
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Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells
The culture of human pluripotent stem cells under defined conditions most commonly relies on the use of Matrigel as a substrate upon which the cells remain in an undifferentiated state. Matrigel is a complex m...
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Adipogenic Differentiation of Human Induced Pluripotent Stem Cells
Human induced pluripotent stem (iPS) cells are promising sources of disease modeling and regenerative medicine. However, differentiation properties of human iPS cells to specific lineages still remain to be fu...
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Morphological Assessments of Traumatic Brain Injury
This chapter reviews documented methods for tracking morphological damage across the neurovascular unit following experimental models of focal or diffuse traumatic brain injury. Sections within this chapter fo...
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Electrophysiological Evaluation of Synaptic Plasticity in Injured CNS
Brain ischemia and injuries often cause alterations in short- and long-term synaptic plasticity, which underlies impaired brain functions, such as learning and memory deficit and epilepsy. Field potential reco...
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Light Microscopic Assessment
Light microscopy is a fundamental and essential assessment for cerebral vasospasm since the degree of cerebral vasospasm has been evaluated by angiography or light microscopy. Its methodology has already been ...
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Cranial Window Assessments in Experimental aSAH in Mice
The chronic cranial window model offers the possibility of a direct in vivo assessment of the cortical surface and pial vasculature in multiple physiological and pathological CNS states. Within the last years,...
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Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells
Although embryonic stem (ES) cells have the regenerative capability of producing any tissue in our body, the development of efficient methods to generate a large number of ES cells with high purity has still r...
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Direct Differentiation of Human Embryonic Stem Cells into Selective Neurons on Nanoscale Ridge/Groove Pattern Arrays
Human embryonic stem cells (hESCs) are pluripotent cells that have the potential to be used for tissue engineering and regenerative medicine. Biochemical and biological agents are widely used to induce hESC di...
