311 Result(s)
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Xun **a
Biochemistry, general
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Article
Expression, Purification and Characterization of a Recombinant β-Glucosidase from Volvariella Volvacea
For the first time, a β-glucosidase gene from the edible straw mushroom, Volvariella volvacea V1-1, has been over-expressed in E. coli. The gene product was purified by chromatography showing a single band on SDS...
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Article
Enhanced Expression and Purification of Membrane Proteins by SUMO Fusion in Escherichia coli
Severe acute respiratory syndrome coronavirus (SARS-CoV) membrane protein and 5-lipoxygenase-activating protein (FLAP) are among a large number of membrane proteins that are poorly expressed when traditional e...
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Article
Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution
A novel strategy to prepare negatively charged and small DNA-containing liposomes after condensation of plasmid DNA by a cationic lipid in deoxycholate micelle environment is described. The average diameter of...
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Article
Expression of Galactose Permease and Pyruvate Carboxylase in Escherichia coli ptsG Mutant Increases the Growth Rate and Succinate Yield under Anaerobic Conditions
In Escherichia coli, disruption of ptsG, which encodes the glucose-specific permease of the phosphotransferase transport system (PTS) protein EIICBGlc, is crucial for high succinate production. This mutation can,...
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Article
Amino-acid Type Identification in 15N-HSQC Spectra by Combinatorial Selective 15N-labelling
The efficiency of cell-free protein synthesis combined with combinatorial selective 15N-labelling provides a method for the rapid assignment of 15N-HSQC cross-peaks to the 19 different non-proline amino-acid type...
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Article
Expression of Galactose Permease and Pyruvate Carboxylase in Escherichia coli ptsG Mutant Increases the Growth Rate and Succinate Yield under Anaerobic Conditions
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Article
Expression and purification of a mutant of human interleukin-2 in Pichia pastoris
Interleukin (IL)-2 is a pharmacologically important cytokine secreted by T-lymphocytes. Recombinant IL-2 (rIL-2) has been modified and produced in many systems. Mass production of rIL-2 is the prerequisite for...
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Article
Over-expression of glucose dehydrogenase improves cell growth and riboflavin production in Bacillus subtilis
Ribulose 5-phosphate is a precursor for riboflavin biosynthesis. Alteration of carbon flow into the pentose phosphate pathway will affect the availability of ribulose 5-phosphate and the riboflavin yield. We h...
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Article
Improved production of ethanol by deleting FPS1 and over-expressing GLT1 in Saccharomyces cerevisiae
To improve ethanol production in Saccharomyces cerevisiae, two yeast strains were constructed. In the mutant KAM-3, the FPS1 gene, which encodes a channel protein responsible for glycerol export, was deleted. The...
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Article
Measurement of dissociation constants of high-molecular weight protein–protein complexes by transferred 15N-relaxation
The use of 15N-relaxation data for determination of the dissociation constant of a protein–protein complex is proposed for the situation where a 15N-labeled protein is bound to an unlabeled protein of high molecu...
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Article
Tissue-driven Hypothesis with Gene Ontology (GO) Analysis
Most of the genes are under selective pressure to maintain their expression levels in the tissues. In a recent study, we have proposed a “tissue-driven” hypothesis stating that the stabilizing constraints on g...
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Article
RNAi and microRNA: breakthrough technologies for the improvement of plant nutritional value and metabolic engineering
This article raises the complex issue of improving plant nutritional value through metabolic engineering and the potential of using RNAi and micro RNA technologies to overcome this complexity, focusing on a fe...
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Article
Cloning and characterization of a thermostable superoxide dismutase from the thermophilic bacterium Rhodothermus sp. XMH10
A superoxide dismutase (SOD) gene was cloned from the thermophilic bacterium Rhodothermus sp. XMH10 for the first time and highly expressed in Escherichia coli. The Rhodothermus sp. XMH10 SOD (RhSOD) gene encodes...
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Article
Strong Genetic Differentiation of Primula sikkimensis in the East Himalaya–Hengduan Mountains
The East Himalaya-Hengduan Mountains region is the center of diversity of the genus Primula, and P. sikkimensis is one of the most common members of the genus in the region. In this study, the genetic diversity a...
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Article
Metabolic profiling by ion mobility mass spectrometry (IMMS)
Ion Mobility Mass Spectrometry (IMMS) was evaluated as an analytical method for metabolic profiling. The specific instrument used in these studies was a direct infusion (DI)-electrospray ionization (ESI)—ambie...
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Article
Gene frequency of five genetic characteristics in six nationalities in Southern Guizhou Province, China
Five characteristics (hair forms, nasal profile, nostril forms, mongoloid fold and upper eyelid fold) were respectively investigated in six nationalities, including the Buyi, Miao, Shui, Maonan, Dong, and Han ...
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Article
Numbat: an interactive software tool for fitting Δχ-tensors to molecular coordinates using pseudocontact shifts
Pseudocontact shift (PCS) effects induced by a paramagnetic lanthanide bound to a protein have become increasingly popular in NMR spectroscopy as they yield a complementary set of orientational and long-range ...
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Article
Molecular characteristics of the tubeworm, Ridgeia piscesae, from the deep-sea hydrothermal vent
Ridgeia piscesae, living around the extremely harsh hydrothermal vent in deep sea, is an ideal model for studying the adaptative mechanism to extreme environment. For insights of its mole...
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Article
Molecular Cloning, Sequencing, and Expression of a l-Glutamine d-Fructose 6-Phosphate Amidotransferase Gene from Volvariella volvacea
Using 3′-RACE and 5′-RACE, we have cloned and sequenced the genomic gene and complete cDNA encoding l-glutamine d-fructose 6-phosphate amidotransferase (GFAT) from the edible straw mushroom, Volvariella volvacea....
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Article
Open AccessBinding of PFOS to serum albumin and DNA: insight into the molecular toxicity of perfluorochemicals
Health risk from exposure of perfluorochemicals (PFCs) to wildlife and human has been a subject of great interest for understanding their molecular mechanism of toxicity. Although much work has been done, the ...
