48 Result(s)

Article

The role of the transcription factor DP in apoptosis

DP1 and DP2 function as binding partners for E2F transcription factors. The association of DP with E2F directly enhances both the DNA binding affinity and the transactivation function of the heterodimer. Targe...

M. R. Hitchens, P. D. Robbins in Apoptosis (2003)

Article

Intra-articular adenoviral-mediated gene transfer of trail induces apoptosis of arthritic rabbit synovium

Rheumatoid arthritis (RA) is an inflammatory autoimmune disease that primarily affects joints. In rheumatoid joints there is extensive synovial proliferation with diseased synovium becoming highly aggressive, ...

Q Yao, S Wang, A Gambotto, J C Glorioso, C H Evans, P D Robbins… in Gene Therapy (2003)

Article

Gene therapy for arthritis

Rheumatoid arthritis is an autoimmune disease with intra-articular inflammation and synovial hyperplasia that results in progressive degradation of cartilage and bone, in severe cases it causes systemic compli...

P D Robbins, C H Evans, Y Chernajovsky in Gene Therapy (2003)

Article

Dendritic cells genetically engineered to express IL-4 exhibit enhanced IL-12p70 production in response to CD40 ligation and accelerate organ allograft rejection

C57BL/10 (B10; H2^b) bone marrow-derived myeloid dendritic cells (DC) propagated in GM-CSF + IL-4 were transduced with r adenoviral (Ad) vectors encoding either control neomycin-resistance gene (Ad-Neo) or murine ...

K Kaneko, Z Wang, S H Kim, A E Morelli, P D Robbins, A W Thomson in Gene Therapy (2003)

Article

Therapeutic and specific antitumor immunity induced by co-administration of immature dendritic cells and adenoviral vector expressing biologically active IL-18

Interleukin-18 is a potent cytokine expressed early in the immune response following cleavage in activated composes. We have investigated the in vivo antitumor effects of intratumoral (i.t.) administration of an ...

F Tanaka, W Hashimoto, P D Robbins, M T Lotze, H Tahara in Gene Therapy (2002)

Article

Inhibition of cartilage destruction by double gene transfer of IL-1Ra and IL-10 involves the activin pathway

The objective of the study was to determine the effects and the molecular background of interleukin-1 receptor antagonist (IL-1Ra) and vIL-10 double gene transfer into human synovial fibroblasts from patients ...

E Neumann, M Judex, F Kullmann, J Grifka, P D Robbins, T Pap, R E Gay… in Gene Therapy (2002)

Article

IL-1Ra and vIL-10 gene transfer using retroviral vectors ameliorates particle-associated inflammation in the murine air pouch model

Objective: This study examined anti-inflammatory gene therapy to ameliorate tissue responses to ultra high molecular weight polyethylene (UHMWPE) particles in the murine air pouch.¶Meth...

S. Yang, B. Wu, L. Mayton, C. H. Evans, P. D. Robbins… in Inflammation Research (2002)

Article

Gene Therapy for Autoimmune Disorders

Although many autoimmune disorders do not have a strong genetic basis, their treatment may nevertheless be improved by gene therapies. Most strategies seek to transfer genes encoding immunomodulatory products ...

C. H. Evans, S. C. Ghivizzani, T. J. Oligino… in Journal of Clinical Immunology (2000)

Article

Genetic enhancement of fracture repair: healing of an experimental segmental defect by adenoviral transfer of the BMP-2 gene

This study evaluated the ability of gene transfer to enhance bone healing. Segmental defects were created surgically in the femora of New Zealand white rabbits. First generation adenoviruses were used as vecto...

A W A Baltzer, C Lattermann, J D Whalen, P Wooley, K Weiss, M Grimm… in Gene Therapy (2000)

Article

TGFβ-1-Gentransfer in Gelenkknorpelzellen

H. D. Möller, F. H. Fu, C. Niyibizi, R. K. Studer, H. J. Georgescu… in Der Orthopäde (2000)

Article

Intra-articular delivery of a herpes simplex virus IL-1Ra gene vector reduces inflammation in a rabbit model of arthritis

To evaluate the use of HSV-based vectors for arthritis gene therapy we have constructed a first-generation, ICP4 deficient, replication defective herpes simplex virus (HSV) vector (S/0−) and a second-generatio...

T Oligino, S C Ghivizzani, D Wolfe, E R Lechman, D Krisky, Z Mi, C H Evans… in Gene Therapy (1999)

Article

Infection of intact human islets by a lentiviral vector

The transfer of genes encoding immunomodulatory proteins to islets can be used to improve islet function, block apoptosis, and inhibit rejection following transplantation. Adenoviral vectors have been shown to...

N Giannoukakis, Z Mi, A Gambotto, A Eramo, C Ricordi, M Trucco, P D Robbins in Gene Therapy (1999)

Article

Gene therapy in transplantation

Facilitation of solid organ and cell transplantation depends on metabolic and immunologic factors that can be manipulated ex vivo and in vivo using gene transfer technology. Vectors have been developed which c...

N Giannoukakis, A W Thomson, P D Robbins in Gene Therapy (1999)

Article

In vitro Transduktion humaner osteoblastärer Zellpopulationen mit retroviralen Vektoren

Einleitung: Interleukin-1 ist ein wichtiger Faktor in der Pathophysiologie der Osteoporose und möglicherweise der periartikulären Knochenresorption bei rheumatoider Arthritis. Die Nutzung von therapeutischen Pro...

A. W. A. Baltzer, J.D. Whalen, T. Muzzonegro… in Zeitschrift für Rheumatologie (1999)

Article

Potent antitumor effects mediated by local expression of the mature form of the interferon-γ inducing factor, interleukin-18 (IL-18)

IL-18 is produced during the acute immune response by macrophages and immature dendritic cells. IL-18 receptors are induced on T cells and NK cells by IL-12 and together they enhance a cellular immune response...

T Osaki, W Hashimoto, A Gambotto, H Okamura, P D Robbins, M Kurimoto… in Gene Therapy (1999)

Article

Co-delivery of T helper 1-biasing cytokine genes enhances the efficacy of gene gun immunization of mice: studies with the model tumor antigen β-galactosidase and the BALB/c Meth A p53 tumor-specific antigen

DNA-based immunization is currently being investigated as a new method for the induction of cellular and humoral immunity directed against viral disease and cancer. In the present study we characterized and co...

T Tüting, A Gambotto, P D Robbins, W J Storkus, A B DeLeo in Gene Therapy (1999)

Article

Adenoviral delivery of CTLA4Ig into myeloid dendritic cells promotes their in vitro tolerogenicity and survival in allogeneic recipients

Dendritic cells (DC) are highly specialized antigen-presenting cells (APC) that initiate and modulate immune responses. They are essential for naive T cell activation, but may also play roles both in central a...

L Lu, A Gambotto, W-C Lee, S Qian, C A Bonham, P D Robbins, A W Thomson in Gene Therapy (1999)

Article

Retrovirally transduced bone marrow stromal cells isolated from a mouse model of human osteogenesis imperfecta (oim) persist in bone and retain the ability to form cartilage and bone after extended passaging

Bone marrow stromal cells isolated from a model of osteogenesis imperfecta (oim) mice, were transduced with a retrovirus (BAG) carrying the LacZ and neo^r genes after passage 21. The transduced cells retained the ...

M Oyama, A Tatlock, S Fukuta, K Kavalkovich, K Nishimura, B Johnstone… in Gene Therapy (1999)

Chapter

Retroviral Vectors

Retroviral vectors represent the most widely used vector system for gene therapy. They are currently in the clinic for treatment of a wide variety of diseases, from cancer to virus infection to arthritis. Alth...

P. D. Robbins in Gene Therapy (1999)

Chapter

Enhancement of Anti-Tumor Immunity by Injection of Fibroblasts Genetically Engineered to Produce IL-12 and to Express CD70

The ability of tumors to grow in the presence of a functional immune system suggests that the host immune mechanisms have failed to detect and eliminate tumor cells. This failure does not result from the absen...

V. Douin-Echinard, P. D. Robbins, M. T. Lotze, G. Favre… in Gene Therapy of Cancer (1998)