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Article
Open AccessThermometric analysis of blood metabolites in ICU patients
Real-time monitoring of patient’s blood metabolites, such as glucose and lactate, could potentially improve surgery and recovery outcomes for patients in surgical and intensive care units. Our enzyme thermomet...
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Article
Open AccessPlant based production of myoglobin - a novel source of the muscle heme-protein
Myoglobin is a heme-protein in the muscle of vertebrates with important functions in the oxygenation of tissues and as a regulator in nitric oxide signaling. Myoglobin from many species is also an important nu...
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Article
Open AccessCorrection to: Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination
The article Pyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination, written by Weihong **e, Leif Bü low, Bin **e, was originally published elec...
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Article
Open AccessPyrroloquinoline quinone glucose dehydrogenase adopted in thermometric analysis for enhancement of glucose determination
A broad measurement range of glucose is often required in clinical analysis, especially for diabetic patients where glucose levels can be very high. Pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) has ...
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Chapter
The Penultimate Tyrosine Residues are Critical for the Genotoxic Effect of Human Hemoglobin
Hemoglobin (Hb) is a potent oxidant outside the erythrocyte. The tyrosines α140 and β145 play an important role in the structure and function of Hb by forming switch and hinge contacts. These carboxy-terminal ...
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Chapter
Characterization of Protein-Protein Interactions in Recombinant Hemoglobin Producing Escherichia coli Cells Using Molecularly Imprinted Polymers
The worldwide blood shortage has generated demands for alternatives to transfusible human blood. One such important option is based on recombinant hemoglobin-based oxygen carriers (rHBOCs). Most efforts have b...
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Article
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere
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Chapter and Conference Paper
Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs)
The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinan...
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Article
In vitro preparation of amelogenin nanoparticles carrying nucleic acids
Amelogenin, a matrix protein involved in biomineralization of enamel, can self-assemble to form nanospheres in a pH-dependent manner. Nucleic acids (single-stranded, double-stranded, and plasmid DNA, as well a...
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Article
Preparation of a portable point-of-care in vitro diagnostic system, for quantification of canine C-reactive protein, based on a magnetic two-site immunoassay
In this study, characterization of the binding kinetics and optimization of a magnetic permeability based point-of-care (POC) immunoassay system for quantification of canine C-reactive protein (cCRP) is descri...
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Chapter
Recombinant Human Hb‐SOD Fusion Proteins
Hemoglobin (Hb) can produce reactive oxygen species, including superoxide anions, which are intrinsically toxic. Superoxide dismutase (SOD) is present in red blood cells (RBCs) and provides important protectio...
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Article
Open AccessUse of human amelogenin in molecular encapsulation for the design of pH responsive microparticles
Proteins can be used in drug delivery systems to improve pharmacological properties of an active substance. Differences in pH between tissues can be utilized in order to achieve a targeted drug release at a sp...
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Article
Direct electrochemistry and bioelectrocatalysis of a class II non-symbiotic plant haemoglobin immobilised on screen-printed carbon electrodes
In this study, direct electron transfer (ET) has been achieved between an immobilised non-symbiotic plant haemoglobin class II from Beta vulgaris (nsBvHb2) and three different screen-printed carbon electrodes bas...
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Article
Metal ion accessibility of histidine-modified superfolder green fluorescent protein expressed in Escherichia coli
Green fluorescent protein (GFP) is frequently utilized for metal ion detection and quantification. To improve the metal binding potential of GFP, three residues (N146, F165, and L201) were substituted to histi...
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Article
An investigation of the peroxidase activity of Vitreoscilla hemoglobin
In order to investigate the ability of the Vitreoscilla hemoglobin (VHb) to act as a peroxidase, the protein was overexpressed in Escerichia coli and purified using a 6xHis-tag. The peroxidase activity of VHb was...
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Article
Enhanced ribosome and tRNA contents in Escherichia coli expressing a truncated Vitreoscilla hemoglobin mutant analyzed by flow field-flow fractionation
The ribosome and tRNA levels of Escherichia coli cells, transformed with a native or mutated Vitreoscilla hemoglobin genes (vhb), were investigated using asymmetrical flow field-flow fractionation (AFFFF). Mutage...
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Article
Selection of Burkholderia pseudomallei protease-binding peptides by phage display
Burkholderia pseudomallei is a causative agent of melioidosis, a fatal community acquired septicemia in Southeast Asia and Northern Australia. A protease has been proposed to be one of the major pathogenic facto...
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Article
Enhanced fructose levels in field grown potato tubers expressing a thermostable glucose isomerase
A thermostable glucose isomerase was expressed in Solanum tuberosum Desirée using the tuber-specific granular-bound starch synthase promoter. The fructose content was substantially increased in microtubers, green...
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Article
Directed Evolution of Vitreoscilla Hemoglobin (VHb) to support the Growth of Escherichia coli under Microaerobic Conditions
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Article
Monitoring of Ribosomes and Subunits in Escherichia Coli During Production of Glucose Isomerase Using Flow Field-Flow Fractionation
Ribosomal components and tRNA of E. coli TG1 producing a recombinant enzyme, glucose isomerase, were monitored by asymmetrical flow field-flow fractionation. The growth temperatures were 20, 30 and 37°C. Resul...
