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Monitoring of Ribosomes and Subunits in Escherichia Coli During Production of Glucose Isomerase Using Flow Field-Flow Fractionation

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Biotechnology Techniques

Abstract

Ribosomal components and tRNA of E. coli TG1 producing a recombinant enzyme, glucose isomerase, were monitored by asymmetrical flow field-flow fractionation. The growth temperatures were 20, 30 and 37°C. Results indicate a correlation between translational capacity and the amount obtained of the recombinant enzyme. Highest yield was obtained when cultivating at 30°C.

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Authors and Affiliations

  1. Technical Analytical Chemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden

    Mikael Nilsson & Karl-Gustav Wahlund

  2. Pure and Applied Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden

    Leif Bülow

Authors
  1. Mikael Nilsson
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  2. Karl-Gustav Wahlund
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  3. Leif Bülow
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Nilsson, M., Wahlund, KG. & Bülow, L. Monitoring of Ribosomes and Subunits in Escherichia Coli During Production of Glucose Isomerase Using Flow Field-Flow Fractionation. Biotechnology Techniques 12, 477–480 (1998). https://doi.org/10.1023/A:1008867508039

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  • DOI: https://doi.org/10.1023/A:1008867508039

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