Abstract
In cyanobacteria, the interactions among pigment–protein complexes are modified in response to changes in light conditions. In the present study, we analyzed excitation energy transfer from the phycobilisome and photosystem II to photosystem I in the cyanobacterium Arthrospira (Spirulina) platensis. The cells were grown under lights with different spectral profiles and under different light intensities, and the energy-transfer characteristics were evaluated using steady-state absorption, steady-state fluorescence, and picosecond time-resolved fluorescence spectroscopy techniques. The fluorescence rise and decay curves were analyzed by global analysis to obtain fluorescence decay-associated spectra. The direct energy transfer from the phycobilisome to photosystem I and energy transfer from photosystem II to photosystem I were modified depending on the light quality, light quantity, and cultivation period. However, the total amount of energy transferred to photosystem I remained constant under the different growth conditions. We discuss the differences in energy-transfer processes under different cultivation and light conditions.
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Abbreviations
- Car:
-
Carotenoid
- Chl:
-
Chlorophyll
- FDAS:
-
Fluorescence decay-associated spectrum (spectra)
- LED:
-
Light-emitting diodes
- PBS:
-
Phycobilisome
- PS:
-
Photosystem
- TRFS:
-
Time-resolved fluorescence spectrum (spectra)
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Acknowledgments
The authors would like to thank Mr. F. Hamada and Ms. A. Teshigahara for their help in measurements and analyses at the early stage of this study. This work was partly supported by a Grant-in-Aid for Scientific Research from JSPS (Nos. 22370017 and 23370013) to SA and a Grant-in-Aid for JSPS Fellows (No. 21A72944) to MY.
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Akimoto, S., Yokono, M., Aikawa, S. et al. Modification of energy-transfer processes in the cyanobacterium, Arthrospira platensis, to adapt to light conditions, probed by time-resolved fluorescence spectroscopy. Photosynth Res 117, 235–243 (2013). https://doi.org/10.1007/s11120-013-9830-5
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DOI: https://doi.org/10.1007/s11120-013-9830-5