Abstract
In this study, a novel adsorbent was prepared by modification of graphite with deep eutectic solvent based on camphor–thymol (by the mole ratio of 1:1) and was utilized as a separation phase for pipette tip micro-solid phase extraction of mitoxantrone from seawater, drug, urine and blood samples. After extraction, mitoxantrone content of samples was determined by spectrophotometry at 260 nm wavelength. Various variables which could affect the extraction, including pH, amount of extracting phase, volumes of sample and eluent, type of eluent, and number of the extraction/ejection cycles of solution and eluent, were evaluated and optimized by both one-variable-at-a-time and response surface methodology methods. Observations indicated that the highest extraction achieved when 200 mg of the adsorbent was employed for 100 µL of sample solution with 7 extraction cycles at pH 7.0. Analyte was desorbed from the adsorbent by 200 µL of methanol (as eluent) after 3 ejection cycles. The linear dynamic range of the technique was between 1 and 1000 µg L−1, and the limit of detection and quantification were 0.2 µg L−1 and 0.7 µg L−1, respectively. Finally, the proposed extraction was successfully employed for the determination of mitoxantrone in various real samples with complicated matrices.
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig1_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig2_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig3_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig4_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig5_HTML.png)
![](http://media.springernature.com/m312/springer-static/image/art%3A10.1007%2Fs13738-023-02951-2/MediaObjects/13738_2023_2951_Fig6_HTML.png)
Similar content being viewed by others
References
Y. Liu, M. Wei, Y. Hu, L. Zhu, J. Du, Sens. Actuators B Chem. 255, 544 (2018)
L.G. Rossato, V.M. Costa, P.G. de Pinho, M.D. Arbo, V. de Freitas, L. Vilain, M.L. de Bastos, C. Palmeira, F. Remião, Arch. Toxicol. 87, 1809 (2013)
M. Enache, E. Volanschi, J. Pharm. Pharmacol. 64, 688 (2012)
L. Slordal, A. Andersen, D.J. Warren, Ther. Drug Monit. 15, 328 (1993)
A.H. Ahmed, A. Ramadan, S.M. Derayea, RSC Adv. 12, 7413 (2022)
R. Tietze, E. Schreiber, S. Lyer, C. Alexiou, J. Biomed. Biotechnol. 1, 103 (2010)
G. An, M.E. Morris, J. Pharm. Biomed. Anal. 51, 750 (2010)
H. Yao, M. Zhang, W. Zeng, X. Zeng, Z. Zhang, Spectrochim. Acta Part A 117, 645 (2014)
C. McLaughlin, D. MacMillan, C. McCardle, W.E. Smith, Anal. Chem. 74, 3160 (2002)
X.Y. Jiang, D. Feng, G.L. Liu, D.H. Fan, W. Wang, Sens. Actuat. B Chem. 232, 276 (2016)
M. Kaykhaii, S.H. Hashemi, Chapter 4: miniaturized solid phase extraction. Emerging Freshwater Pollutants, 49 (2022).
S.H. Hashemi, M. Kaykhaii, A.J. Keikha, E. Mirmoradzehi, Spectrochim. Acta A Mol. Biomol. Spectrosc. 213, 218 (2019)
P.M. Chełstowska, M. Kaykhaii, J.P. Wasylka, M. Guardia, J. Mol. Liq. 365, 120158 (2022)
A.R. Harifi-Mood, M. Sarafrazi, J. Environ. Chem. Eng. 11, 109177 (2023)
K. Xu, Y. Wang, H. Zhang, Q. Yang, X. Wei, P. Xu, Y. Zhou, Microchim. Acta 184, 4133 (2017)
M.R. Rezaei Kahkha, M. Kaykhaii, G. Sargazi, B.R. Kahkha, Anal. Methods 11, 6168 (2019)
S.H. Hashemi, N. Naruie, J. Anal. Chem. 76, 563 (2021)
S.H. Hashemi, F. Keykha, Anal. Methods 11, 5405 (2019)
S.H. Hashemi, M. Kaykhaii, A.J. Keikha, Z. Sajjadi, Marine Poll. Bull. 137, 306 (2018)
S.H. Hashemi, M. Kaykhaii, A.J. Keikha, Z. Sajjadi, M. Mirmoghaddam, Analyst 144, 3525 (2019)
M. Tamandani, S.H. Hashemi, J. Food Compos. 112, 104658 (2022)
Acknowledgements
We gratefully acknowledge the financial support received from the Research Council of Chabahar Maritime University.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
Authors declare that they have no conflicts of interest.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Esmailiun, M., Hashemi, S.H. Deep eutectic solvent-doped graphite as an efficient adsorbent for pipette tip micro-solid phase extraction of mitoxantrone from seawater, drug, urine and blood samples prior to its spectrophotometric determination. J IRAN CHEM SOC 21, 689–698 (2024). https://doi.org/10.1007/s13738-023-02951-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13738-023-02951-2