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Development of Electrochemical Biosensor for miR204-Based Cancer Diagnosis

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Abstract

With increase in cancer burden worldwide and poor survival rates due to delayed diagnosis, it is pertinent to develop a device for early diagnosis. We report an electrochemical biosensor for quantification of miRNA-204 (miR-204) biomarker that is dysregulated in most of the cancers. The proposed methodology uses the gold nanoparticles-modified carbon screen-printed electrode for immobilization of single-stranded DNA probe against miR-204. Colloidal gold nanoparticles were synthesized using l-glutamic acid as reducing agent. Nanoparticles were characterized by UV–visible spectroscopy and transmission electron microscopy. Spherical gold nanoparticles were of 7–28 nm in size. Biosensor fabricated using these nanoparticles was characterized by cyclic voltammetry after spiking 0.1 fg/mL–0.1 µg/mL of miR-204 in fetal bovine serum. Response characteristics of the miR-204 biosensor displayed high sensitivity of 8.86 µA/µg/µL/cm2 with wide detection range of 15.5 aM to 15.5 nM. The low detection limit makes it suitable for early diagnosis and screening of cancer.

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Acknowledegements

Shilpa is thankful to Jaypee Institute of Information Technology Noida, India, for providing research assistance to complete this project. The authors are also thankful to the Advanced Instrumentation and Research Facility (AIRF), Jawaharlal Nehru University, for extending the facility for transmission electron microscopy analysis.

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  1. Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, U.P., India

    Shilpa Gundagatti & Sudha Srivastava

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  1. Shilpa Gundagatti
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Correspondence to Sudha Srivastava.

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Gundagatti, S., Srivastava, S. Development of Electrochemical Biosensor for miR204-Based Cancer Diagnosis. Interdiscip Sci Comput Life Sci 14, 596–606 (2022). https://doi.org/10.1007/s12539-022-00508-0

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