Abstract
MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (ν) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.
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Data obtained during the present study are available from the corresponding author on request.
References
Valsami-Jones E, Lynch I (2015) How safe are nanomaterials? Science 80:388–389
Baig N, Kammakakam I, Falath W (2021) Nanomaterials: a review of synthesis methods, properties, recent progress, and challenges. Mater Adv 2(6):1821–1871
Zhang Z, Karimi-Maleh H (2023) In situ synthesis of label-free electrochemical aptasensor-based sandwich-like AuNPs/PPy/Ti3C2Tx for ultrasensitive detection of lead ions as hazardous pollutants in environmental fluids. Chemosphere 324:138302
Zhang ZX, Karimi-Maleh H (2023) Label-free electrochemical aptasensor based on gold nanoparticles/titanium carbide MXene for lead detection with its reduction peak as index signal. Adv Compos Hybrid Mater 6(68)
Karimi-Maleh H et al (2023) State-of-art advances on removal, degradation and electrochemical monitoring of 4-aminophenol pollutants in real samples: a review. Environ Res 222:115338
Karimi-Maleh H et al (2022) Determination of D&C Red 33 and Patent Blue V Azo dyes using an impressive electrochemical sensor based on carbon paste electrode modified with ZIF-8/g-C3N4/Co and ionic liquid in mouthwash and toothpaste as real samples. Food Chem Toxicol 162:112907
Mehmandoust M et al (2021) Three-dimensional porous reduced graphene oxide decorated with carbon quantum dots and platinum nanoparticles for highly selective determination of azo dye compound tartrazine. Food Chem Toxicol 158:112698
Karimi-Maleh H et al (2023) Calf thymus ds-DNA intercalation with pendimethalin herbicide at the surface of ZIF-8/Co/rGO/C3N4/ds-DNA/SPCE; a bio-sensing approach for pendimethalin quantification confirmed by molecular docking study. Chemosphere 332:138815
Ameen F et al (2023) A novel atropine electrochemical sensor based on silver nano particle-coated Spirulina platensis multicellular blue-green microalga. Chemosphere 324:138180
Dabirifar Z et al (2022) Design of Co-Sn bimetallic nanoalloys as electrocatalyst for alkaline methanol oxidation reaction: Exploring the effect of electroactivation process. Fuel 319:123727
Karimi F et al (2022) Polyaniline-manganese ferrite supported platinum-ruthenium nanohybrid electrocatalyst: synergizing tailoring toward boosted ethanol oxidation reaction. Top Catal 65(5–6):716–725
Karimi-Maleh H et al (2022) Nanochemistry approach for the fabrication of Fe and N co-decorated biomass-derived activated carbon frameworks: a promising oxygen reduction reaction electrocatalyst in neutral media. J Nanostruct Chem 12(3):429–439
Jafarzadeh H et al (2022) Hydrogen production via sodium borohydride hydrolysis catalyzed by cobalt ferrite anchored nitrogen-and sulfur co-doped graphene hybrid nanocatalyst: artificial neural network modeling approach. Chem Eng Res Des 183:557–566
Karaman O (2022) Three-dimensional graphene network supported nickel-cobalt bimetallic alloy nanocatalyst for hydrogen production by hydrolysis of sodium borohydride and develo** of an artificial neural network modeling to forecast hydrogen production rate. Chem Eng Res Des 181:321–330
Karaman C et al (2022) Congo red dye removal from aqueous environment by cationic surfactant modified-biomass derived carbon: Equilibrium, kinetic, and thermodynamic modeling, and forecasting via artificial neural network approach. Chemosphere 290:133346
Aboli E, Jafari D, Esmaeili H (2020) Heavy metal ions (lead, cobalt, and nickel) biosorption from aqueous solution onto activated carbon prepared from Citrus limetta leaves. Carbon Lett 30(6):683–698
Bae J, Hong JY (2021) Fabrication of nitrogen-doped porous carbon nanofibers for heavy metal ions removal. Carbon Lett 31(6):1339–1347
Mubarak MF et al (2022) Adsorption of heavy metals and hardness ions from groundwater onto modified zeolite: batch and column studies. Alex Eng J 61(6):4189–4207
**a C et al (2023) Spotlighting the boosted energy storage capacity of CoFe2O4/Graphene nanoribbons: a promising positive electrode material for high-energy-density asymmetric supercapacitors. Energy 270:126914
Goei R et al (2022) Development of nitrogen-decorated carbon dots (NCDs) thermally conductive film for windows application. Carbon Lett 32(4):1065–1072
Hosseinzadeh K et al (2021) Effect of two different fins (longitudinal-tree like) and hybrid nano-particles (MoS2–TiO2) on solidification process in triplex latent heat thermal energy storage system. Alex Eng J 60(1):1967–1979
Kandathil V et al (2021) A green and sustainable cellulosic-carbon-shielded Pd-MNP hybrid material for catalysis and energy storage applications. J Nanostruct Chem 11(3):395–407
Magne TM et al (2022) Graphene and its derivatives: understanding the main chemical and medicinal chemistry roles for biomedical applications. J Nanostruct Chem 12(5):693–727
Ameen F et al (2022) Antioxidant, antibacterial and anticancer efficacy of Alternaria chlamydospora-mediated gold nanoparticles. Appl Nanosci 13(3)
Moghadam NCZ et al (2022) Nickel oxide nanoparticles synthesis using plant extract and evaluation of their antibacterial effects on Streptococcus mutans. Bioprocess Biosyst Eng 45(7):1201–1210
Megarajan S et al (2022) Synthesis of N-myristoyltaurine stabilized gold and silver nanoparticles: assessment of their catalytic activity, antimicrobial effectiveness and toxicity in zebrafish. Environ Res 212(Pt A):113159
Ayranci R et al (2017) Carbon based nanomaterials for high performance optoelectrochemical systems. ChemistrySelect 2(4):1548–1555
Sen B et al (2021) Bimetallic palladium-cobalt nanomaterials as highly efficient catalysts for dehydrocoupling of dimethylamine borane (vol 45, pg 3569, 2020). Int J Hydrogen Energy 46(39):20797–20798
Aygun A et al (2022) Highly active PdPt bimetallic nanoparticles synthesized by one-step bioreduction method: characterizations, anticancer, antibacterial activities and evaluation of their catalytic effect for hydrogen generation. Int J Hydrogen Energy 6666–6679
Goksu H et al (2021) Bimetallic nanomaterials for direct alcohol fuel cells. Nanomaterials for direct alcohol fuel cells. Elsevier, pp 145–156
Akin M et al (2021) Carbon-based nanomaterials for alcohol fuel cells. Nanomaterials for direct alcohol fuel cells. Elsevier, pp 319–336
Babudurai M et al (2021) Mechanical activation of TiO2/Fe2O3 nanocomposite for arsenic adsorption: effect of ball-to-powder ratio and milling time. J Nanostruct Chem 11(4):633–633
Ameen F, Dawoud T, AlNadhari S (2021) Ecofriendly and low-cost synthesis of ZnO nanoparticles from Acremonium potronii for the photocatalytic degradation of azo dyes. Environ Res 202:111700
Erduran V et al (2022) Functionalized carbon material-based electrochemical sensors for day-to-day applications. Functionalized nanomaterial-based electrochemical sensors. Woodhead Publishing, pp 97–111
Akocak S et al (2017) One-pot three-component synthesis of 2-Amino-4H-Chromene derivatives by using monodisperse Pd nanomaterials anchored graphene oxide as highly efficient and recyclable catalyst. Nano-Struct Nano-Objects 11:25–31
Ehtesabi H (2020) Carbon nanomaterials for salivary-based biosensors: a review. Mater Today Chem 17:100342
Kadam PM et al (2011) Enhanced electrochromic performance of f-MWCNT-WO3 composite. Electrochim Acta 58:556–561
Yang C et al (2015) Recent trends in carbon nanomaterial-based electrochemical sensors for biomolecules: a review. Anal Chim Acta 887:17–37
Irfan M et al (2014) Surface modification and performance enhancement of nano-hybrid f-MWCNT/PVP90/PES hemodialysis membranes. J Membr Sci 467:73–84
Ermis N et al (2023) Recent advantage in electrochemical monitoring of gallic acid and kojic acid: a new perspective in food science. J Food Measure Charact 2023
Karthik R et al (2017) A highly sensitive and selective electrochemical determination of non-steroidal prostate anti-cancer drug nilutamide based on f-MWCNT in tablet and human blood serum sample. J Colloid Interface Sci 487:289–296
Hwa KY, Sharma TSK (2020) Nano assembly of NiFe spheres anchored on f-MWCNT for electrocatalytic reduction and sensing of nitrofurantoin in biological samples. Sci Rep 10(1):12256
Baghayeri M et al (2014) Multi-walled carbon nanotubes decorated with palladium nanoparticles as a novel platform for electrocatalytic sensing applications. RSC Adv 4(91):49595–49604
Haleem A et al (2021) Biosensors applications in medical field: a brief review. Sensors Int 2:100100
Falkowski P, Lukaszewski Z, Gorodkiewicz E (2021) Potential of surface plasmon resonance biosensors in cancer detection. J Pharm Biomed Anal 194:113802
Kaya SI et al (2020) Carbon-based ruthenium nanomaterial-based electroanalytical sensors for the detection of anticancer drug Idarubicin. Sci Rep 10(1):11057
Bekmezci M et al (2022) Biofunctionalization of functionalized nanomaterials for electrochemical sensors. Functionalized nanomaterial-based electrochemical sensors. Woodhead Publishing, pp 55–69
Sadeghi H et al (2022) Electrochemical determination of vitamin B6 in water and juice samples using an electrochemical sensor amplified with NiO/CNTs and Ionic liquid. Int J Electrochem Sci 15:10488–10498
Tabrizi M et al (2022) Reduce graphene oxide/Fe3O4 nanocomposite biosynthesized by sour lemon peel; using as electro-catalyst for fabrication of vanillin electrochemical sensor in food products analysis and anticancer activity. Top Catal 65(5–6):726–732
Arjmandi J et al (2022) Sudan I monitoring as a hazardous azo dye using an electroanalytical tool amplified with NiO/SWCNTs-ionic liquid catalysts. Chemosphere 309(Pt 1):136673
Vatandost E et al (2020) Antioxidant, antibacterial and anticancer performance of reduced graphene oxide prepared via green tea extract assisted biosynthesis. ChemistrySelect 5(33):10401–10406
Nair RR et al (2008) Fine structure constant defines visual transparency of graphene. Science 320(5881):1308–1308
Savk A et al (2019) Multiwalled carbon nanotube-based nanosensor for ultrasensitive detection of uric acid, dopamine, and ascorbic acid. Mater Sci Eng C-Mater Biol Appl 99:248–254
Arikan K et al (2022) Glucose nano biosensor with non-enzymatic excellent sensitivity prepared with nickel-cobalt nanocomposites on f-MWCNT. Chemosphere 291(Pt 3):132720
Dalla Pozza M et al (2017) Magneto-elastic biosensors: Influence of different thiols on pathogen capture efficiency. Mater Sci Eng C-Mater Biol Appl 75:629–636
Zhang KY et al (2021) Machine learning-reinforced noninvasive biosensors for healthcare. Adv Healthcare Mater 10(17):e2100734
Tüylek Z (2017) Biyosensörler ve Nanoteknolojik Etkileşim. BEU J Sci 6:71–80
Ambros V (2004) The functions of animal microRNAs. Nature 431(7006):350–355
Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136(2):215–233
Liu B et al (2016) iMiRNA-PseDPC: microRNA precursor identification with a pseudo distance-pair composition approach. J Biomol Struct Dyn 34(1):223–235
**ao RL, Li C, Chai BF (2015) miRNA-144 suppresses proliferation and migration of colorectal cancer cells through GSPT1. Biomed Pharmacother 74:138–144
Wei LS et al (2018) Clinical utilization of serum-or plasma-based miRNAs as early detection biomarkers for pancreatic cancer: a meta-analysis up to now. Medicine 97(35):e12132
Baradaran B, Shahbazi R, Khordadmehr M (2019) Dysregulation of key microRNAs in pancreatic cancer development. Biomed Pharmacother 109:1008–1015
He BX et al (2020) miRNA-based biomarkers, therapies, and resistance in Cancer. Int J Biol Sci 16(14):2628–2647
Luo QQ et al (2022) Functional mechanism and clinical implications of miR-141 in human cancers. Cell Signal 95:110354
Zhao G et al (2013) miRNA-141, downregulated in pancreatic cancer, inhibits cell proliferation and invasion by directly targeting MAP4K4. Mol Cancer Ther 12(11):2569–2580
Ma LX et al (2019) The miR-141/neuropilin-1 axis is associated with the clinicopathology and contributes to the growth and metastasis of pancreatic cancer. Cancer Cell Int 19:248
Pothipor C et al (2021) A highly sensitive electrochemical microRNA-21 biosensor based on intercalating methylene blue signal amplification and a highly dispersed gold nanoparticles/graphene/polypyrrole composite. Analyst 146(8):2679–2688
Rafiee-Pour HA, Behpour M, Keshavarz M (2016) A novel label-free electrochemical miRNA biosensor using methylene blue as redox indicator: application to breast cancer biomarker miRNA-21. Biosens Bioelectron 77:202–207
Kilic T et al (2015) Electrochemical detection of a cancer biomarker mir-21 in cell lysates using graphene modified sensors. Electroanalysis 27(2):317–326
Kaplan M et al (2017) A novel method for sensitive microRNA detection: electropolymerization based do**. Biosens Bioelectron 92:770–778
Hong CY et al (2013) Ultrasensitive electrochemical detection of cancer-associated circulating microRNA in serum samples based on DNA concatamers. Biosens Bioelectron 50:132–136
Yang LL et al (2021) Electrochemical sensor based on Prussian blue/multi-walled carbon nanotubes functionalized polypyrrole nanowire arrays for hydrogen peroxide and microRNA detection. Microchimica Acta 188(1):2
Kunovsky L et al (2018) The use of biomarkers in early diagnostics of pancreatic cancer. Can J Gastroenterol Hepatol 2018:5389820
Wong W et al (2020) BRCA mutations in pancreas cancer: spectrum, current management, challenges and future prospects. Cancer Manage Res 12:2731–2742
Hong SM et al (2020) Three-dimensional visualization of cleared human pancreas cancer reveals that sustained epithelial-to-mesenchymal transition is not required for venous invasion. Mod Pathol 33(4):639–647
Na’ara S, Amit M, Gil Z (2019) L1CAM induces perineural invasion of pancreas cancer cells by upregulation of metalloproteinase expression. Oncogene 38(4):596–608
Ji D et al (2020) Smartphone-based square wave voltammetry system with screen-printed graphene electrodes for norepinephrine detection. Smart Mater Med 1:1–9
Hasan S et al (2019) Advances in pancreatic cancer biomarkers. Oncol Rev 13(1):69–76
Gayral M et al (2014) MicroRNAs as emerging biomarkers and therapeutic targets for pancreatic cancer. World J Gastroenterol 20(32):11199–11209
Jelski W, Mroczko B (2019) Biochemical diagnostics of pancreatic cancer—present and future. Clin Chim Acta 498:47–51
Zhu HY et al (2018) Pancreatic cancer: challenges and opportunities. BMC Med 16(1):214
Wu WR et al (2018) Rising trends in pancreatic cancer incidence and mortality in 2000–2014. Clin Epidemiol 10:789–797
Nehlsen AD, Goodman KA (2021) Controversies in radiotherapy for pancreas cancer. J Surg Oncol 123(6):1460–1466
Yamanaka K, Vestergaard MC, Tamiya E (2016) Printable electrochemical biosensors: a focus on screen-printed electrodes and their application. Sensors 16(10):1761
Malvano F et al (2016) Impedimetric label-free immunosensor on disposable modified screen-printed electrodes for ochratoxin A. Biosensors-Basel 6(3):33
Smart A et al (2020) Screen -printed carbon based biosensors and their applications in agri-food safety. Trac-Trends Anal Chem 127:115898
Hughes G et al (2016) Recent advances in the fabrication and application of screen-printed electrochemical (Bio)sensors based on carbon materials for biomedical, agri-food and environmental analyses. Biosensors-Basel 6(4):50
Jewell E, Philip B, Greenwood P (2016) Improved manufacturing performance of screen printed carbon electrodes through material formulation. Biosensors-Basel 6(3):30
Sotiropoulou S et al (2003) Novel carbon materials in biosensor systems. Biosens Bioelectron 18(2–3):211–215
Xu D et al (2020) Lost miR-141 and upregulated TM4SF1 expressions associate with poor prognosis of pancreatic cancer: regulation of EMT and angiogenesis by miR-141 and TM4SF1 via AKT. Cancer Biol Ther 21(4):354–363
Sun JM, Zhang YB (2019) LncRNA XIST enhanced TGF-beta 2 expression by targeting miR-141–3p to promote pancreatic cancer cells invasion. Biosci Rep 39(7):BSR20190332
Bartel DP (2007) MicroRNAs: genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281–297, 2004). Cell 131(4):11–29
Karimi S et al (2012) A nanocomposite based biosensor for cholesterol determination. Anal Methods 4(10):3225–3231
Zaine IS et al (2014) Study on dispersion and characterization of functionalized mwcnts prepared by wet oxidation. In: Applied mechanics and materials. Trans Tech Publications Ltd, pp 8–13
Dhall S, Vaidya G, Jaggi N (2014) Joining of broken multiwalled carbon nanotubes using an electron beam-induced deposition (EBID) technique. J Electron Mater 43(9):3283–3289
Ulus R et al (2016) Functionalized multi-walled carbon nanotubes (f-MWCNT) as highly efficient and reusable heterogeneous catalysts for the synthesis of acridinedione derivatives. ChemistrySelect 1(13):3861–3865
Rojas JV et al (2016) Facile radiolytic synthesis of ruthenium nanoparticles on graphene oxide and carbon nanotubes. Mater Sci Eng B-Adv Funct Solid-State Mater 205:28–35
Khodadoust A et al (2022) A ratiometric electrochemical DNA-biosensor for detection of miR-141. Microchimica Acta 189(6):213
Tran HV et al (2013) Label-free and reagentless electrochemical detection of microRNAs using a conducting polymer nanostructured by carbon nanotubes: application to prostate cancer biomarker miR-141. Biosens Bioelectron 49:164–169
Wang LL et al (2018) Nitrogen-doped hollow carbon nanospheres for high-energy-density biofuel cells and self-powered sensing of microRNA-21 and microRNA-141. Nano Energy 44:95–102
Tian R, Li YJ, Bai JW (2019) Hierarchical assembled nanomaterial paper based analytical devices for simultaneously electrochemical detection of microRNAs. Anal Chim Acta 1058:89–96
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This study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK) TEYDEB 1507 Grant No 7191026.
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Bekmezci, M., Bayat, R., Akin, M. et al. Modified screen-printed electrochemical biosensor design compatible with mobile phones for detection of miR-141 used to pancreatic cancer biomarker. Carbon Lett. 33, 1863–1873 (2023). https://doi.org/10.1007/s42823-023-00545-9
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DOI: https://doi.org/10.1007/s42823-023-00545-9