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Determination of 12 quinolones in honey by vortex-assisted dispersive liquid liquid microextraction performed in syringe based on deep eutectic solvent combine with ultra performance liquid chromatography-mass spectrometry

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Abstract

A novel vortex-assisted dispersive liquid–liquid microextraction method based on deep eutectic solvent (VA–DLLME–DES) completed in syringe has been established to extract 12 quinolones from honey. This method is an improvement of traditional DLLME. In this work, the green DES prepared with heptanoic acid and thymol was used for extractant rather than traditional organic extraction solvent. Without centrifugation, the phases were separated in the way of removing aqueous phase by pushing the syringe and extractant phase was collected for ultra performance liquid chromatography-mass spectrometry (UPLC–MS) analysis. The stability of DES and some experimental parameters (type, molar ratios and volume of extraction solvent, type and volume of dispersant, vortex time and effect of pH and NaCl) were evaluated. In the range of 2–100 ng/mL, the proposed method showed good linearities (r2 ranged from 0.996 to 0.999). The extraction recoveries were obtained in the range of 75.01%–117.05% and relative standard deviations were less than 13.83% for inter- (n = 6) and intra-day (n = 3) precisions. The limits of detection and quantification were 3 ng g−1 and 10 ng g−1, respectively. The developed method with the advantages of simple operation, short extraction time and less consumption organic solvent provides a technical idea for green, simple and time-saving sample preparation methods.

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Acknowledgements

The authors gratefully thank the National Natural Science Foundation of China (31372482), Project of Tian** dairy (mutton sheep) industry technology system innovation team construction (ITTCRS2021000) and Project of Tian** “131” innovative talent team (20180318) for financial support.

Funding

This work was supported by the National Natural Science Foundation of China (31372482), Project of Tian** dairy (mutton sheep) industry technology system innovation team construction (ITTCRS2021000) and Project of Tian** “131” innovative talent team (20180318).

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Authors and Affiliations

  1. Tian** Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tian** Agricultural University, Tian**, 300392, China

    Yixia Wang, Linyan Yang, Chang Liu, Hongyu Wang, Daowen Li, Wei Zhang, Liuan Li & Cun Li

  2. China Animal Health and Epidemiology Center, Qingdao, 266032, Shandong, China

    Sijun Zhao & Cui** Song

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  1. Yixia Wang
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  7. Wei Zhang
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Correspondence to Cui** Song or Cun Li.

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Wang, Y., Zhao, S., Yang, L. et al. Determination of 12 quinolones in honey by vortex-assisted dispersive liquid liquid microextraction performed in syringe based on deep eutectic solvent combine with ultra performance liquid chromatography-mass spectrometry. Eur Food Res Technol 248, 263–272 (2022). https://doi.org/10.1007/s00217-021-03878-9

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