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Experimental strategies to improve drug-target identification in mass spectrometry-based thermal stability assays
Mass spectrometry (MS)-based thermal stability assays have recently emerged as one of the most promising solutions for the identification of...
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Chromodomain on Y-like 2 (CDYL2) implicated in mitosis and genome stability regulation via interaction with CHAMP1 and POGZ
Histone H3 trimethylation on lysine 9 (H3K9me3) is a defining feature of mammalian pericentromeres, loss of which results in genome instability. Here...
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Experimental validation of stability and applicability of Start Growth Time method for high-throughput bacterial ecotoxicity assessment
Ecotoxicity assessments based on bacteria as model organisms are widely used for routine toxicity screening because it has the advantages of...
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LC–MS based case-by-case analysis of the impact of acidic and basic charge variants of bevacizumab on stability and biological activity
The present study investigates the impact of charge variants on bevacizumab's structure, stability, and biological activity. Five basic and one...
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Identification of cerebrospinal fluid biomarkers for parkinsonism using a proteomics approach
The aim of our study was to investigate cerebrospinal fluid (CSF) tryptic peptide profiles as potential diagnostic biomarkers for the discrimination...
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Mass spectrometric profiling of tryptic digests of trifluoroethanol extracts from core needle biopsies of breast cancer tissues is a viable sample screening tool for biomarker discovery
Application of classifying limited sampling biopsies by matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) based mass spectral...
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Pressure-Assisted Thermal Sterilization and Storage Stability of Avocado Puree in High Barrier Polymeric Packaging
The main objective of this study was to evaluate the feasibility of develo** shelf stable avocado puree processed with pressure-assisted thermal...
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Identification of apolipoprotein E-derived amyloid within cholesterol granulomas of leopard geckos (Eublepharis macularius)
Apolipoprotein E (ApoE) is involved in cholesterol transport among cells and also plays an important role in amyloid formation, co-depositing with...
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Improved Identification of Protease Cleavage Sites by In-gel Reductive Dimethylation
A critical step in the functional characterization of proteases is the identification of physiologically relevant substrates, which often starts with...
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LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX
LASS2 functions as a tumor suppressor in hepatocellular carcinoma (HCC), the most common type of primary liver cancer, but the underlying mechanism...
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Effect of N-glycosylation on secretion, stability, and biological activity of recombinant human interleukin-3 (hIL-3) in Pichia pastoris
Human interleukin-3 (hIL-3) is a clinically important cytokine used to treat hematological malignancies, bone marrow transplantation, cytopenias, and...
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Polymerase iota (POLI) confers radioresistance of esophageal squamous cell carcinoma by regulating RAD51 stability and facilitating homologous recombination
Radiotherapy resistance is an important and urgent challenge in the clinical management of esophageal squamous carcinoma (ESCC). However, the factors...
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Novel Selective Quantification of Zinpentraxin Alfa Biotherapeutic in the Presence of Endogenous Isomer in Plasma Samples of Idiopathic Pulmonary Fibrosis Patients Using Immunoaffinity LC–MS
Idiopathic pulmonary fibrosis (IPF) is a progressive fatal interstitial lung disease that affects three million patients worldwide and currently...
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Development of a ternary cyclodextrin–arginine–ciprofloxacin antimicrobial complex with enhanced stability
Designing useful functionalities in clinically validated, old antibiotics holds promise to provide the most economical solution for the global lack...
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Reciprocal interaction between SIRT6 and APC/C regulates genomic stability
SIRT6 is an NAD + -dependent deacetylase that plays an important role in mitosis fidelity and genome stability. In the present study, we found that...
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Tryptic Map** Based Structural Insights of Endo-1, 4-β-Xylanase from Thermomyces lanuginosus VAPS-24
An endo-1,4-β-xylanase, XynA, from Thermomyces lanuginosus VAPS-24, was purified to homogeneity and exhibited a molecular mass of approximately...
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Multidimensional separation and analysis of alpha-1-acid glycoprotein N-glycopeptides using high-field asymmetric waveform ion mobility spectrometry (FAIMS) and nano-liquid chromatography tandem mass spectrometry
Bottom-up nLC-MS/MS-based glycoprotein mass spectrometry workflows rely on the generation of a mixture of non-glycosylated and glycosylated peptides...
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A Strong Cation Exchange Chromatography Protocol for Examining N-Terminal Proteoforms
Especially in eukaryotes, the N-terminal acetylation status of a protein reveals translation initiation sites and substrate specificities and...
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Differential effects of SUMO1 and SUMO2 on circadian protein PER2 stability and function
Posttranslational modification (PTM) of core circadian clock proteins, including Period2 (PER2), is required for proper circadian regulation. PER2...
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Immunoaffinity extraction followed by enzymatic digestion for the isolation and identification of proteins employing automated μSPE reactors and mass spectrometry
This work describes a novel automated and rapid method for bottom-up proteomics combining protein isolation with a micro-immobilised enzyme reactor...
