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Tryptic Map** Based Structural Insights of Endo-1, 4-β-Xylanase from Thermomyces lanuginosus VAPS-24

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Abstract

An endo-1,4-β-xylanase, XynA, from Thermomyces lanuginosus VAPS-24, was purified to homogeneity and exhibited a molecular mass of approximately 20 kDa. The protein sequence of XynA was found to be similar to those of other Thermomyces lanuginosus derived xylanases and, as a result, could be used as a model enzyme for understanding the protein structure–activity relationship and facilitating protein engineering to design enzyme variants with desirable properties. Therefore, this xylanase will be an attractive candidate for applications in the biofuel and fine chemical industries for the degradation of xylans in steam pre-treated biomass.

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Acknowledgements

We would like to thank Dr. Stoyan Stoychev (CSIR, South Africa) for identifying the xylanase used in this study using tryptic map**/MS. The authors are also grateful for the financial support received for this study from the National Research Foundation (NRF) of South Africa and Rhodes University (Sandisa Imbewu). Any opinion, findings and conclusions or recommendations expressed in this material are those of the author(s), and therefore the NRF does not accept liability in regard thereto. VK is thankful to UGC New Delhi, India, for awarding him the Junior Research Fellowship [F.17-63/2008 (SA-I)]. PS acknowledges the support from the Department of Biotechnology, Government of India (Grant No. BT/PR27437/BCE/8/1433/2018), SERB, Department of Science and Technology, Government of India (DST Fast Track Grant No. SR/FT/LS-31/2012) and the infrastructural support from Department of Science and Technology, Government of India through a FIST Grant (Grant No. 1196 SR/FST/LS-I/2017/4).

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Authors and Affiliations

  1. Enzyme Science Programme (ESP), Department of Biochemistry and Microbiology, Rhodes University, Grahamstown, Eastern Cape, 6140, South Africa

    Brian N. Mathibe, Samkelo Malgas, Layla Radosavljevic & Brett I. Pletschke

  2. Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India

    Vishal Kumar & Pratyoosh Shukla

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  1. Brian N. Mathibe
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Correspondence to Pratyoosh Shukla or Brett I. Pletschke.

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Mathibe, B.N., Malgas, S., Radosavljevic, L. et al. Tryptic Map** Based Structural Insights of Endo-1, 4-β-Xylanase from Thermomyces lanuginosus VAPS-24. Indian J Microbiol 60, 392–395 (2020). https://doi.org/10.1007/s12088-020-00879-2

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