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Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites
Phagosome maturation is critical for immune defense, defining whether ingested material is destroyed or converted into antigens. Sec22b regulates...
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Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution
Cells such as macrophages and neutrophils can internalize a diverse set of particulate matter, illustrated by bacteria and apoptotic bodies through...
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Quantitative Spatio-temporal Analysis of Phagosome Maturation in Live Cells
Phagocytosis and phagosome maturation are central processes to the development of the innate and adaptive immune response. Phagosome maturation is a...
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Kinetics of phagosome maturation is coupled to their intracellular motility
Immune cells degrade internalized pathogens in phagosomes through sequential biochemical changes. The degradation must be fast enough for effective...
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Assessing the Phagosome Proteome by Quantitative Mass Spectrometry
The process of phagocytosis involves a series of defined steps, including the formation of a new intracellular organelle, i.e., the phagosome, and...
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Microscopy-Based Tracking and Quantification Methods to Study Phagosome Resolution
Phagosome resolution is a newly defined, terminal stage in the process of phagocytosis. During this phase, phagolysosomes are fragmented into smaller...
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Phagocytosis and Phagosomes Methods and Protocols
This volume explores updated and entirely new experimental approaches used to investigate phagocytosis and phagosome maturation. In order to aid in...
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Legionella effectors SidC/SdcA ubiquitinate multiple small GTPases and SNARE proteins to promote phagosomal maturation
Protein ubiquitination is one of the most important posttranslational modifications (PTMs) in eukaryotes and is involved in the regulation of almost...
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Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes
Professional phagocytic cells, such as macrophages, ingest large particles into a specialized endocytic compartment, the phagosome, which eventually...
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Transcriptome analysis of Schizothorax oconnori (Cypriniformes: Cyprinidae) oocytes: The role of K+ in promoting yolk globule fusion and regulating oocyte maturation
Schizothorax oconnori ( S. oconnori ) is an economically important fish in Tibet. Oocyte maturation is a physiological process that is of great...
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Dissecting Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Murine Dendritic Cells
Phagosomal pattern recognition receptor signaling promotes phagosome maturation and additional immune pathways such as proinflammatory cytokine...
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Phagocytosis
One hundred years have passed since the death of Élie Metchnikoff (1845–1916). He was the first to observe the uptake of particles by cells and...
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Biochemical and Microscopic Analyses for Sphingolipids and Its Related Molecules in Phagosomes
Glycosphingolipids (GSLs) form GSL-enriched microdomains, together with sphingomyelin (SM), cholesterol, glycosylphosphatidylinositol (GPI)-anchored...
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Filamentous Bacteria as Targets to Study Phagocytosis
Filamentous targets are internalized via phagocytic cups that last for several minutes before closing to form a phagosome. This characteristic offers...
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An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates
Phagosomes are formed when phagocytic cells take up large particles, and they develop into phagolysosomes where the particles are degraded. The...
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Mycobacterium tuberculosis and its clever approaches to escape the deadly macrophage
Mycobacterium tuberculosis (Mt.b ), a deadly disease causer, is a facultative parasite. This microorganism has developed several methods to defend...
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Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting
The engulfment of “self” and “non-self” particles by immune and non-immune cells is crucial for maintaining homeostasis and combatting infection....
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epHero – a tandem-fluorescent probe to track the fate of apoptotic cells during efferocytosis
The efficient removal of apoptotic cells via efferocytosis is critical for maintaining optimal tissue function. This involves the binding and...
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Protrudin-mediated ER-endosome contact sites promote phagocytosis
During phagocytosis, endosomes both contribute with membrane to forming phagosomes and promote phagosome maturation. However, how these vesicles are...
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Proteomics analysis of methionine enkephalin upregulated macrophages against infection by the influenza-A virus
Macrophages have a vital role in phagocytosis and antiviral effect against invading influenza viruses. Previously, we found that methionine...
