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Quantitative Analysis of Nucleoside Triphosphate Pools in Mouse Muscle Using Hydrophilic Interaction Liquid Chromatography Coupled with Tandem Mass Spectrometry Detection
Defects in deoxyribonucleoside triphosphate (dNTP) metabolism are associated with a number of mitochondrial DNA (mtDNA) depletion syndromes (MDS)....
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Comparison of reversed-phase, hydrophilic interaction, and porous graphitic carbon chromatography columns for an untargeted toxicometabolomics study in pooled human liver microsomes, rat urine, and rat plasma
IntroductionUntargeted metabolomics studies are expected to cover a wide range of compound classes with high chemical diversity and complexity. Thus,...
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Liquid Chromatography and Capillary Electrophoresis Analysis of 2AA-Labeled O-Glycans
To reveal O-glycan structures in mucins, it is necessary to release covalently bound O-glycans from the polypeptide backbone and derivatize to a form...
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Chromatography
This chapter covers the principle, working, and operations of different types of chromatographic techniques like paper, thin layer, column...
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Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy
Type I lipoteichoic acid (LTA) is a glycerol phosphate polymer found in the cell envelope of diverse Gram-positive bacteria. The glycerol phosphate...
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Application of Liquid Chromatography for the Analysis of Flavonoids in Food: An Overview
Flavonoids are a known group of organic compounds found in plants, where they act as dyes and natural fungicides. Together with the food of plant...
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Liquid Chromatography-Mass Spectrometry for Clinical Metabolomics: An Overview
Metabolomics is a discipline that offers a comprehensive analysis of metabolites in biological samples. In the last decades, the notable evolution in...
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Liquid–liquid phase separation of H3K27me3 reader BP1 regulates transcriptional repression
BackgroundBromo-adjacent homology-plant homeodomain domain containing protein 1 (BP1) is a reader of histone post-translational modifications in...
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Utilizing Skyline to analyze lipidomics data containing liquid chromatography, ion mobility spectrometry and mass spectrometry dimensions
Lipidomics studies suffer from analytical and annotation challenges because of the great structural similarity of many of the lipid species. To...
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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) variants currently represent the best tools to tackle the...
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Adhesion-related properties of silver birch (Betula Pendula Roth) wood as affected by hydrophilic extraction
For the utilization of silver birch ( Betula pendula R oth ) in load-bearing engineered wood products (EWPs), reliable bonding in production is a...
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Untargeted Analysis of Short-Chain Peptides in Urine Samples Short Peptides Analysis
Short-chain peptides have attracted increasing attention in different research fields, including biomarker discovery, but also a well-known...
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9-Fluorenylmethyl Chloroformate Labeling for O-Glycan Analysis
Structural analysis of O-glycans from mucins and characterization of the interaction of these glycans with other biomolecules are essential for a...
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Advanced analysis of oligomeric proanthocyanidins: latest approaches in liquid chromatography and mass spectrometry based analysis
Proanthocyanidins (PAC) are an important and widely spread class of natural products with various bioactivities. The analytical evaluation of...
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A High-Throughput HILIC-MS-Based Metabolomic Assay for the Analysis of Polar Metabolites
Mass spectrometry (MS)-based metabolomics approaches have been used for characterizing the metabolite content and composition of biological samples...
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Comprehensive Analysis of Plant Lipids Using Sub-2-μm Particle CO2-Based Chromatography Coupled to Mass Spectrometry
Lipids play an essential role in plants, and historically manipulating their levels and composition has been an important target for metabolic...
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The Synergistic Activity and Interaction Mechanism of Linoleic Acid and a Bioactive Pentapeptide (Tyr-Val-Pro-His-Trp) from Walnut Meal in the Treatment of Alzheimer’s Disease
The walnut meal is a by-product of walnut oil pressing, which contains a large amount of protein and active compounds. The Walnut protein was...
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Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Metabolic Flux Analysis
Accurate quantification of mass isotopolog distribution (MID) of intracellular metabolites is a key requirement for 13C metabolic flux analysis...
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Metabolomics: Going Deeper, Going Broader, Going Further
Metabolomics is a continuously dynamic field of research that is driven by demanding research questions and technological advances alike. In this...
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The CNK–HYP scaffolding complex promotes RAF activation by enhancing KSR–MEK interaction
The RAS–MAPK pathway regulates cell proliferation, differentiation and survival, and its dysregulation is associated with cancer development. The...
