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The effects of sesame protein isolate and transglutaminase enzyme on the quality characteristics of gluten-free batter and cake
Manufacturers are interested in producing high-quality gluten-free (GF) bakery products, and many have turned to additives to improve their texture,...
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Software for Predicting Binding Free Energy of Protein–Protein Complexes and Their Mutants
Protein–protein binding affinity prediction is important for understanding complex biochemical pathways and to uncover protein interaction networks....
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Physicochemical properties and angiotensin-I converting enzyme inhibitory activity of lipid-free ribbon fish (Lepturacanthus savala) protein hydrolysate
The study aims at removal of lipid from ribbon fish protein hydrolysate (FPH) to enhance the protein content and analyse its physicochemical and...
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Paper-based uric acid assay in whole blood samples by Zn2+ protein precipitation and enzyme-free colorimetric detection
Uric acid (UA) is an important biomarker, as a high concentration in blood can lead to gout and further renal syndrome. Although several...
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Cell-Free Protein Synthesis of Metalloproteins
Metalloproteins, proteins containing metal atoms or clusters within their structures, are critical for various biological functions across all...
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Research progress of multi-enzyme complexes based on the design of scaffold protein
Multi-enzyme complexes designed based on scaffold proteins are a current topic in molecular enzyme engineering. They have been gradually applied to...
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Cell-free Protein Synthesis Systems: Increasing their Performance and Applications
The Escherichia coli cell-free protein synthesis system can now be used for various proteins that need special requirements, such as disulfide bond...
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Cell-Free Display Techniques for Protein Evolution
Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of...
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A Review of Background and Application of ATPSs in Protein and Enzyme Extraction
This review article provides an overview of the fundamental background and application of aqueous two-phase systems (ATPSs) in protein and enzyme...
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Tag-free protein modification by lipoate ligase A: exploring substrate tolerance
This study delves into the functional intricacies of lipoate ligase A (LplA), an enzyme showing great promise in bioconjugation due to its unique...
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A High-Affinity Methyl-CpG-Binding Protein for Endonuclease-Free and Label-Free DNA Methyltransferase Activity Detection
DNA methyltransferase (MTase) activity detection has received increasing attention as a promising biomarker and therapeutic target. However, most of...
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Oligomerization of Hydroperoxide Lyase, a Novel P450 Enzyme in Plants
The oligomeric state of fatty acid hydroperoxide lyase (HPL), of molar mass ∼55 kDa is uncertain and it has been reported as a trimer or tetramer...
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Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis
Technical advances in biotechnology have greatly accelerated the development of bottom-up synthetic biology. Unlike top-down approaches, bottom-up...
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Immobilization of lipase enzyme onto herbal silver nanoparticles and examination of biochemical parameters of immobilized enzyme
Nanoparticles are useful for immobilization due to their size and physical properties. The present study aimed to synthesize herbal silver...
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Effect of enzyme hydrolysis on the functionality, protein quality, and flavour profile of lentil and chickpea protein isolates
The overarching goal of this research was to investigate the effect of trypsin hydrolysis on the functional properties, protein quality, and flavour...
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Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology
Cell-free systems for the in vitro production of proteins have revolutionized the synthetic biology field. In the last decade, this technology is...
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Towards harmonization of directly measured free 25-hydroxyvitamin D using an enzyme-linked immunosorbent assay
The majority of circulating 25-hydroxyvitamin D (25(OH)D) is protein bound and perhaps less available than the free fraction of 25(OH)D; therefore,...
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Development of in vitro lycopene biosynthesis from geranyl pyrophosphate employing cell-free protein synthesis
Lycopene is a compound classified as carotenoid, also known as tetraterpenoids, and its high antioxidative capabilities make demand in pharmaceutical...
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A Cell-free Protein Synthesis Method for the Detection of Heavy Metal Ions Using a Personal Glucose Meter
Heavy metal pollution of water can pose a serious threat to public health. Because pollution can occur at multiple stages of water distribution,...
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Enzyme-Assisted Extraction of Plant Proteins
Enzyme-assisted extraction (EAE) is an environmentally friendly green processing technique used to aid protein extraction from different plant...