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Hybrid Genome Assembly of Short and Long Reads in Galaxy
Galaxy is a web browser-based data analysis platform that is widely used in biology. Public Galaxy instances allow the analysis of data and...
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Microbial Genome Sequencing and Assembly Using Nanopore Sequencers
Microbial genomes are typically several million base pairs in length and are relatively easy to sequence and assemble into a single chromosome, given...
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De Novo Genome Assembly of Japanese Black Cattle as Model of an Economically Relevant Animal
A genetic analysis of Japanese Black cattle using short reads and guided by the reference genome from Western breeds would miss the structural...
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The impact of applying various de novo assembly and correction tools on the identification of genome characterization, drug resistance, and virulence factors of clinical isolates using ONT sequencing
Oxford Nanopore sequencing technology (ONT) is currently widely used due to its affordability, simplicity, and reliability. Despite the advantage ONT...
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Self-Assembly DNA-Conjugated Polymer for DNA Immobilization on Chip
DNA chips are extensively used for genome and transcriptome analyses in the post-genomic sequence era. A self-assembly technique can be applied to...
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Reference-based assembly of chloroplast genome from leaf transcriptome data of Pterocarpus santalinus
Chloroplast genome sequencing is an essential tool to understand genome evolution and phylogenetic relationship. The available methods for...
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In Vivo DNA Assembly Using the PEDA Method
Simple and efficient DNA assembly methods have been widely used in synthetic biology. Here, we provide the protocol for the recently developed PEDA...
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Genome Editing Using CRISPR
Genome editing is an indispensable technique utilized in life science and medicine. Development of genome editing tools with high efficiency and...
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Genome Editing Using CRISPR
Genome editing is an indispensable technique utilized in life science and medicine. Development of genome editing tools with high efficiency and...
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CRISPR-Cas9-Mediated Genome Editing in Paenibacillus polymyxa
In recent years, the clustered regularly interspaced palindromic repeats-Cas (CRISPR-Cas) technology has become the method of choice for precision...
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Probiogenomics and Genome Annotation in Bifidobacteria and Lactobacilli
Traditionally, the process of selecting probiotics involved conducting both in vitro and in vivo trials to assess characteristics such as resistance,...
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Gene Nanovector for Genome Therapy
Gene therapy is a potential approach to deal with complicated diseases that are now untreatable. A critical step in the effective gene therapy is the...
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Genome Editing to Improve Nutrition Status of Crop Plants
Genome editing technologies are being adopted globally to improve the nutrient quality of different fruits, vegetables, and staple or oil crops....
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Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDA
Genome sequencing of small species, such as those of meinofauna, can be challenging due to the extremely low input of genomic DNA. While nanopore...
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Chromosome-level assembly and analysis of Camelina neglecta: a novel diploid model for Camelina biotechnology research
Camelina neglecta is a new diploid Brassicaceae species, which has great research value because of its close relationship with the hexaploid oilseed...
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Draft genome sequence of 'Candidatus Phytoplasma asteris,’ strain SW86 associated with sandal spike disease (SSD)
The sandal spike disease (SSD), related to ‘Ca . Phytoplasma asteris’ (Aster Yellows group), poses a significant threat to Indian sandalwood ( Santalum...
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Targeted Genome Editing of Virulent Pseudomonas Phages Using CRISPR-Cas3
The vast number of unknown phage-encoded ORFan genes and limited insights into the genome organization of phages illustrate the need for efficient...
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The Complete Chloroplast Genome Sequence of Staphylea holocarpa (Staphyleaceae)
Staphylea holocarpa (Hemsley 1895) is an ornamental deciduous shrub or tree in the family Staphyleaceae. As the shortage of the wild resources, S....
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Cryptic enzymatic assembly of peptides armed with β-lactone warheads
Nature has evolved biosynthetic pathways to molecules possessing reactive warheads that inspired the development of many therapeutic agents,...
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Fig Tree Genome and Diversity
The common fig (Ficus carica L.) is one of the most remarkable fruit species of the Mediterranean area. Its adaptive potentialities to various...