Probiogenomics and Genome Annotation in Bifidobacteria and Lactobacilli

Abstract

Traditionally, the process of selecting probiotics involved conducting both in vitro and in vivo trials to assess characteristics such as resistance, adherence, and physiological functions. However, further research is needed to fully understand the metabolic capabilities and safety aspects of probiotics. One promising approach for gaining a better understanding of existing and potential probiotics is probiogenomics, which involves studying the genetic makeup of these microorganisms to uncover their health-promoting qualities and safety attributes. In particular, the use of Bifidobacteria and Lactobacilli in functional foods has shown promise in promoting host health, yet the precise molecular mechanisms behind their effects are not completely known. Recent advancements in genome sequencing have provided valuable insights into the genetic adaptations of these bacteria to the gastrointestinal tract and their interactions with the host. Next-generation sequencing (NGS) has made it possible to gather genome-wide data, greatly improving genome annotation efforts. Annotation tasks rely on tools for extracting information from nucleotide sequences, aiding our comprehension of life, evolution, and the diagnosis of genetic disorders. This chapter covers structural and functional annotations, comparative techniques, and visualization tools contributed by the scientific community. Besides these, the chapter covers quality control measures, the need for re-annotations, and the future of this field.