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Development of Fluorescent Immunochromatographic Test Strip for Qualitative and Quantitative Detection of Zearalenone
Zearalenone (ZEN), which is usually generated by Fusarium species, is common contamination of corn. ZEN induces estrogen effects and disrupts the...
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Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(ε-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations
The construction of a rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection is described. We report for the first time a novel one-pot...
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Preparation of Luciferase-fused Peptides for Immunoassay of Amyloid Beta
An immunoassay, such as the enzyme-linked immunosorbent assay (ELISA), is an analytical method that utilizes the interaction of antigens and...
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Handheld pH meter–assisted immunoassay for C-reactive protein using glucose oxidase–conjugated dendrimer loaded with platinum nanozymes
A simple and feasible pH meter–based immunoassay is reported for detection of C-reactive protein (CRP) using glucose oxidase (GOD)–conjugated...
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Sensitive immunoassay of cardiac troponin I using an optimized microelectrode array in a novel integrated microfluidic electrochemical device
A sensitive and portable microfluidic electrochemical array device (μFED) was developed for the immunoassay of trace amounts of human cardiac...
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Bimodal size distribution immuno-quantum dots for fluorescent western blotting assay with high sensitivity and extended dynamic range
A highly sensitive quantum dot (QD)–based western blot assay with extended dynamic range was developed. Bimodal size distribution QD (BQ)...
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Photothermal enhanced fluorescence quenching of Tb-norfloxacin for ultrasensitive human epididymal 4 detection
A fluorescence quenching enhanced immunoassay has been developed to achieve ultrasensitive recognition of human epididymal 4 (HE4) modifying the...
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Immunoassay based on urease-encapsulated metal–organic framework for sensitive detection of foodborne pathogen with pH meter as a readout
The potential of enzyme-encapsulated metal–organic framework (MOF) as an antibody label for the construction of enzyme-linked immunosorbent assay...
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Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor
Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To...
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Distance-dependent visual fluorescence immunoassay on CdTe quantum dot–impregnated paper through silver ion-exchange reaction
A paper-based visual fluorescence immunoassay is presented for the detection of matrix metalloproteinase-7 (MMP7) that is related to renal cancer....
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Fluorescence polarization immunoassay for the determination of diclofenac in wastewater
Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was...
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Ultrasensitive, high-throughput, and rapid simultaneous detection of SARS-CoV-2 antigens and IgG/IgM antibodies within 10 min through an immunoassay biochip
COVID-19 is now a severe threat to global health. Facing this pandemic, we developed a space-encoding microfluidic biochip for high-throughput,...
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One-step hydrothermal synthesis of WS2 quantum dots as fluorescent sensor for sensitive and selective recognition of hemoglobin and cardiac biomarker myoglobin
Transition metal dichalcogenide (TMD) dots exhibit excellent photoluminescence performance due to the quantum confinement effect and edge effect, and...
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A highly sensitive point-of-care detection platform for Salmonella typhimurium by integrating magnetic enrichment and fluorescent CsPbBr3@SiO2
AbstractA platform was designed based on Fe 3 O 4 and CsPbBr 3 @SiO 2 for integrated magnetic enrichment-fluorescence detection of Salmonella typhimurium ,...
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Combined antibodies against internalins A and B proteins have potential application in immunoassay for detection of Listeria monocytogenes
Listeria monocytogenes is a food-borne bacterium that causes listeriosis upon the ingestion of contaminated food. Traditional methods to detect L....
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Development of a quantum dot–based lateral flow immunoassay with high reaction consistency to total aflatoxins in botanical materials
Total aflatoxins (AFTs) are an important safety indicator for botanical materials, but at present, rapid detection technology for AFTs is seldom...
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Fluorescent turn-on assay of C-type natriuretic peptide using a molecularly imprinted ratiometric fluorescent probe with high selectivity and sensitivity
A novel molecularly imprinted ratiometric fluorescent probe was fabricated by simple sol–gel polymerization for selective and sensitive assay of...
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Development of a phage display-mediated immunoassay for the detection of vascular endothelial growth factor
Because of the critical role of vascular endothelial growth factor (VEGF) in angiogenesis and its significantly increased serum levels in early...
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Assays for the Screening and Characterization of Tau Aggregation Inhibitors
Aggregation of tau protein is a pathological hallmark of Alzheimer’s disease and other neurodegenerative tauopathies. Inhibition of tau aggregation... -
A fluorescent and colorimetric dual-channel sensor based on acid phosphatase–triggered blocking of internal filtration effect
A colorimetric and fluorescent dual-channel detection method for acid phosphatase (ACP) activity has been constructed, based on the internal...