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Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor

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Abstract

Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o-phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.

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Funding

The authors received financial support from the National Key R&D Program of China (grant number 2017YFC1601205) and the National Natural Science Foundation of China (NSFC, no. 31871888).

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  1. Yonghong Zha and **aolei Liu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Zoonoses Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, People’s Republic of China

    Yonghong Zha, **aolei Liu, Pan Hu, Shiying Lu, Honglin Ren, Zengshan Liu, Yansong Li & Yu Zhou

  2. College of Animal Sciences, Yangtze University, **gzhou, 434023, People’s Republic of China

    **ongyan Liang, Yuying Yang & Yu Zhou

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  1. Yonghong Zha
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  2. **aolei Liu
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  5. Honglin Ren
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Correspondence to Yansong Li or Yu Zhou.

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Yonghong Zha declares that there is no conflict of interest. **aolei Liu declares that there is no conflict of interest. Pan Hu declares that there is no conflict of interest. Shiying Lu declares that there is no conflict of interest. Honglin Ren declares that there is no conflict of interest. Zengshan Liu declares that there is no conflict of interest. **ongyan Liang declares that there is no conflict of interest. Yuying Yang declares that there is no conflict of interest. Yansong Li declares that there is no conflict of interest. Yu Zhou declares that there is no conflict of interest.

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Zha, Y., Liu, X., Hu, P. et al. Alkaline Phosphatase–Triggered Immunoassay Based on Fluorogenic Reaction for Sensitive Detection of Acetochlor, Metolachlor, and Propisochlor. Food Anal. Methods 13, 1008–1016 (2020). https://doi.org/10.1007/s12161-020-01706-6

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