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Fetal Thymic Organ Culture and Negative Selection
Negative selection removes potentially harmful T cell precursors from the conventional T cell pool. This process can involve the induction of...
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Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies
Fetal thymic organ culture (FTOC) provides a method for analyzing T cell development in a physiological context outside the animal. This technique...
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A guide to thymic selection of T cells
The thymus is an evolutionarily conserved organ that supports the development of T cells. Not only does the thymic environment support the...
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Studying T Cell Development in Neonatal and Adult Thymic Slices
T cell development occurs in the thymus and is coordinated temporally and spatially within the highly complex thymic microenvironment. Therefore, T...
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Revelations in Thymic Epithelial Cell Biology and Heterogeneity from Single-Cell RNA Sequencing and Lineage Tracing Methodologies
Thymic epithelial cells (TECs) make up the thymic microenvironments that support the generation of a functionally competent and self-tolerant T-cell...
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Direct presentation of inflammation-associated self-antigens by thymic innate-like T cells induces elimination of autoreactive CD8+ thymocytes
Upregulation of diverse self-antigens that constitute components of the inflammatory response overlaps spatially and temporally with the emergence of...
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Perinatal thymic-derived CD8αβ-expressing γδ T cells are innate IFN-γ producers that expand in IL-7R–STAT5B-driven neoplasms
The contribution of γδ T cells to immune responses is associated with rapid secretion of interferon-γ (IFN-γ). Here, we show a perinatal thymic wave...
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Cystathionine γ-lyase-derived H2S negatively regulates thymic egress via allosteric inhibition of sphingosine-1-phosphate lyase
Thymic egress is a crucial process for thymocyte maturation, strictly regulated by sphingosine-1-phosphate lyase (S1PL). Recently, cystathionine...
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Primary Culture of Immunological Cells
This chapter begins with a brief description of the mechanisms associated with maintaining immunity (protection against disease-causing pathogens) in...
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Thymic origins of autoimmunity—lessons from inborn errors of immunity
During their intrathymic development, nascent T cells are empowered to protect against pathogens and to be operative for a life-long acceptance of...
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The changing landscape of immune cells in the fetal mouse testis
Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord...
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Mesenchymal stromal cells in the thymus
The microenvironment of the thymus is composed of a group of stromal cells that include endoderm-derived thymic epithelial cells (TECs) and...
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Potential role of mesenchymal stem cells in T cell aging
Immunosenescence occurs with progressive age. T cell aging is manifested by immunodeficiency and inflammation. The main mechanisms are thymic...
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Mice Deficient in NOX2 Display Severe Thymic Atrophy, Lymphopenia, and Reduced Lymphopoiesis in a Zymosan-Induced Model of Systemic Inflammation
Patients with chronic granulomatous disease (CGD) who have mutated phagocyte NADPH oxidase are susceptible to infections due to reduced reactive...
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Insm1 regulates mTEC development and immune tolerance
The expression of self-antigens in medullary thymic epithelial cells (mTECs) is essential for the establishment of immune tolerance, but the...
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Induction of Human T Cell Development In Vitro with OP9-DL4-7FS Cells Expressing Human Cytokines
For nearly a generation now, OP9-DL1 and OP9-DL4 cells have provided an efficient and reliable cell system to generate T cells from mouse and human...
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Humanized Severe Combined Immunodeficient (SCID) Mouse Models for Varicella-Zoster Virus Pathogenesis
Varicella-zoster virus (VZV) is a human-restricted virus, which raises obstacles to research. The strict human tropism limits knowledge about its...
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Contributions of Age-Related Thymic Involution to Immunosenescence and Inflammaging
Immune system aging is characterized by the paradox of immunosenescence (insufficiency) and inflammaging (over-reaction), which incorporate two sides...
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Leaving no one behind: tracing every human thymocyte by single-cell RNA-sequencing
The thymus is the primary organ for T-cell development, providing an essential microenvironment consisting of the appropriate cytokine milieu and...
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Mannosylated glycans impair normal T-cell development by reprogramming commitment and repertoire diversity
T-cell development ensures the formation of diverse repertoires of T-cell receptors (TCRs) that recognize a variety of antigens. Glycosylation is a...
