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Early Development of Innate Lymphoid Cells
Innate lymphoid cells (ILCs) are transcriptionally and functionally similar to T cells but lack adaptive antigen receptors. They play critical roles...
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The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells
The following protocol describes the generation of microglia cells from human-induced pluripotent stem cells (hiPSCs) using commercially available...
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Measurement of Metabolic Alteration in Immune Cells Under Hypoxia
The hypoxic microenvironment in solid tumors affects the metabolism of tumor cells and infiltrating immune cells, which aids in robust tumor growth...
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In Vitro Experimental Approach for Studying Human Pulmonary Artery Smooth Muscle Cells and Endothelial Cells Proliferation and Migration
Pulmonary arterial hypertension (PAH) is a severe vascular disease characterized by persistent precapillary pulmonary hypertension, leading to right...
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Studying Synaptic Integration of Glioma Cells into Neural Circuits
Nervous system activity regulates homeostasis, development, and plasticity of the brain. Certain glial cell populations regulate neuronal activity by...
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Glial Cells During the Life Cycle
Astrocytes, oligodendrocytes, and oligodendrocyte precursor cells have a lifelong close relationship with each other and with neurons. Radial glia...
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Tumorigenic Potential of Cancer Stem Cells In Vivo
Cancer is a nightmare disease due to the tumor heterogeneity resulting in the requirement of multiple treatments. The cell plasticity will lead to...
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Redirecting Human Conventional and Regulatory T Cells Using Chimeric Antigen Receptors
The adaptive immune system exhibits exquisite specificity and memory and is involved in virtually every process in the human body. Redirecting...
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Bioenergetics of Axon Integrity and Its Regulation by Oligodendrocytes and Schwann Cells
Axons are long slender portions of neurons that transmit electrical impulses to maintain proper physiological functioning. Axons in the central...
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Markers and makers of NKT17 cells
Invariant natural killer T ( i NKT) cells are thymus-generated innate-like αβ T cells that undergo terminal differentiation in the thymus. Such a...
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Specification of Granule Cells and Purkinje Cells
Granule cells and Purkinje cells are the major populations of neurons in the cerebellum. Their specification depends on a combination of regional...
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Autophagy in Muscle Stem Cells
Muscle stem cellsMuscle stem cell, also known as satellite cellsSatellite cell, are responsible for the regenerative capacity of adult muscle tissue...
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Differentiation Potential of Cancer Stem Cells In Vitro
Cancer stem cells play an indispensable role in the development and progression of cancer forming the tumor microenvironment. This microenvironment...
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Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs)
This protocol describes a simple and an economical method for isolation of endothelial cells from human umbilical vein. Umbilical cord is easily...
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Purification Process for a Secreted Protein Produced in Insect Cells
The Baculovirus Expression Vector System (BEVS) is used with cultured insect cells to produce a wide variety of heterologous proteins, which can be...
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Nonviral Platform for Expression of Recombinant Protein in Insect Cells
Nonviral transfection has been used to express various recombinant proteins, therapeutics, and virus-like particles (VLP) in mammalian and insect...
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Self-renewal Potential of Cancer Stem Cells
Self-renewal potential is an important characteristic for cancer stem cells (CSCs) to maintain the cancer tissues as well as for the stem cells to...
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Mammalian Cells, Tissues and Organ Culture: Applications
In today’s world, mammalian cell cultures are used to understand various physiological and pathophysiological cell signaling events related to normal...
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Role of Immune Cells in the Tumor Microenvironment
Immune cells of different lineages in the tumor microenvironment (TME) play essential roles in tumorigenesis. Some cells act against the tumor, while...