Abstract
Self-renewal potential is an important characteristic for cancer stem cells (CSCs) to maintain the cancer tissues as well as for the stem cells to maintain the tissues. Depending on this potential CSCs are thought to exhibit many characteristics associated with malignancy followed by chemo- and radio-resistance due to their dormancy leading to tumor recurrence. The self-renewal in normal stem or progenitor cells appears dysregulated in CSCs resulting in the continuous expansion of tumor mass. Therefore, tumor spheres are widely used to analyze the self-renewal capability of CSCs. This method will provide a novel and practical platform for the screening of promising anti-CSC agents.
Here in the current chapter, we demonstrate the method culturing of self-renewing CSCs in tumor spheres, in suspension and in hanging drops. Furthermore, we will demonstrate the method to evaluate sphere-forming potential by the frequency as the extreme limiting dilution analysis (ELDA).
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Afify, S.M., Seno, M. (2023). Self-renewal Potential of Cancer Stem Cells. In: Methods in Cancer Stem Cell Biology. Springer, Singapore. https://doi.org/10.1007/978-981-99-1331-2_11
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DOI: https://doi.org/10.1007/978-981-99-1331-2_11
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