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The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells

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Stem Cell-Based Neural Model Systems for Brain Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2683))

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Abstract

The following protocol describes the generation of microglia cells from human-induced pluripotent stem cells (hiPSCs) using commercially available kits by StemCell Technologies. This protocol consists of three major steps: (1) Differentiation of hematopoietic precursor cells, (2) Microglia differentiation, and (3) Microglia maturation. Assays are described to characterize hematopoietic precursor cells and mature microglia.

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References

  1. Wurm J, Konttinen H, Andressen C et al (2021) Microglia development and maturation and its implications for induction of microglia-like cells from human iPSCs. Int J Mol Sci 22:3088

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  2. Abud E, Ramirez R, Martinez E et al (2017) iPSC-derived human microglia-like cells to study neurological diseases. Neuron 94(2):278–293

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  3. Guttikonda S, Sikkema L, Tchieu J et al (2021) Fully defined human pluripotent stem cell-derived microglia and tri-culture system model C3 production in Alzheimer’s disease. Nat Neurosci 24:343–354

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Acknowledgements

This work was supported by grants from National Institutes of Health (R21AG077697 and P20GM119943 [Subaward 7137296] to Y.A.H), Alzheimer’s Association Strategic Fund (ABA-22-965518 to Y.A.H.), the Graduate Award in Brain Science from Brown’s Carney Institute (to K.C), and the Postdoctoral Fellowship Program in Alzheimer’s Disease Research from the BrightFocus Foundation (A2021002F to B.A.). Y.A.H. is a GFL Translational Professor from Center for Translational Neuroscience at the Brown Institute for Translational Sciences.

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Authors and Affiliations

  1. Department of Molecular Biology, Cell Biology and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA

    Mikael Lehoux, Kevin Connolly, Benedetta Assetta & Yu-Wen Alvin Huang

Authors
  1. Mikael Lehoux
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  2. Kevin Connolly
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  3. Benedetta Assetta
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  4. Yu-Wen Alvin Huang
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Corresponding author

Correspondence to Yu-Wen Alvin Huang .

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Editors and Affiliations

  1. Department of Molecular Biology, Cell Biology, and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA

    Yu-Wen Alvin Huang

  2. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA

    ChangHui Pak

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Lehoux, M., Connolly, K., Assetta, B., Huang, YW.A. (2023). The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_6

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  • DOI: https://doi.org/10.1007/978-1-0716-3287-1_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3286-4

  • Online ISBN: 978-1-0716-3287-1

  • eBook Packages: Springer Protocols

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