21 Result(s)
within
Zhe** Yu
Biochemistry, general
Biotechnology
Microbiology
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Article
Genomic basis for the unique phenotype of the alkaliphilic purple nonsulfur bacterium Rhodobaca bogoriensis
Although several species of purple sulfur bacteria inhabit soda lakes, Rhodobaca bogoriensis is the first purple nonsulfur bacterium cultured from such highly alkaline environments. Rhodobaca bogoriensis strain L...
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Article
Correction to: Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria
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Article
Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria
We describe a new species of purple sulfur bacteria (Chromatiaceae, anoxygenic phototrophic bacteria) isolated from a microbial mat in the sulfidic geothermal outflow of a hot spring in Rotorua, New Zealand. This...
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Article
Quantitative proteomic analysis reveals that serine/threonine kinase is involved in Streptococcus suis virulence and adaption to stress conditions
The eukaryotic-type serine/threonine kinase of Streptococcus suis serotype 2 (SS2) performs critical roles in bacterial pathogenesis. In this study, isobaric tags for relative and absolute quantification (iTRA...
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Article
Blastochloris tepida, sp. nov., a thermophilic species of the bacteriochlorophyll b-containing genus Blastochloris
A new taxon is created for the thermophilic purple nonsulfur bacterium previously designated as Rhodopseudomonas strain GI. Strain GI was isolated from a New Mexico (USA) hot spring microbial mat and grows optima...
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Article
Construction of a plasmid interspecific transfer system in Bacillus species with the counter-selectable marker mazF
Bacillus sp. strains as attractive hosts for the production of heterologous secretory proteins usually play important roles in bio-industry. However, low transformation efficiency of exogenous plasmids limited th...
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Article
Roseovarius lacus sp. nov., isolated from Yuncheng Saline Lake, China
Strain GSS12T, a Gram-negative, aerobic, non-flagellated, ovoid- to rod-shaped (0.5–0.7 × 0.9–3.0 µm) bacterium, was isolated from Yuncheng Saline Lake, China. Growth occurred with 0.5–16.0 % (w/v) NaCl (optimum ...
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Article
High level extracellular production of a truncated alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Escherichia coli by the optimization of induction condition and fed-batch fermentation
To improve the extracellular production of alkaline β-mannanase from alkaliphilic Bacillus sp. N16-5 in Escherichia coli, two truncated recombinant mannanases (32a-ManAR2 and 22b-ManAR2) were obtained. Compared w...
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Article
A method to label biological molecules with dsDNA coated gold nanoparticles
We described a new method to label biological molecules using gold nanoparticles (GNPs) and double stranded DNA. Researchers can conveniently label their own samples with GNPs using this method. The label is b...
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Source identification of bacterial and viral pathogens and their survival/fading in the process of wastewater treatment, reclamation, and environmental reuse
Pathogenic safety is drawing wide concern in water reclamation and reuse. In order to elucidate survive/fade of pathogens during the processes of wastewater treatment and reclamation, general indicators (fecal...
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Article
Bacillus thermophilum sp. nov., isolated from a microbial fuel cell
A novel thermophilic, Gram-staining positive bacterium, designated DX-2T, was isolated from the anode biofilm of a microbial fuel cell. Cells of the strain were oxidase positive, catalase positive, facultative an...
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Article
Pseudomonas oryzae sp. nov. isolated from a paddy soil in South China
A Gram-staining-negative, rod-shaped and motile with several polar flagellums bacterium, designated WM-3T, was isolated from a rice paddy soil in South China. Growth occurred with 0–3.0 % (w/v) NaCl (optimum 2.0 ...
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Article
Analysis of the bacterial communities associated with different drinking water treatment processes
A drinking water plant was surveyed to determine the bacterial composition of different drinking water treatment processes (DWTP). Water samples were collected from different processing steps in the plant (i.e...
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Article
Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens
An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in d...
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Article
Estimation of pullulan by hydrolysis with pullulanase
A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethyl...
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Article
Could nested PCR be applicable for the study of microbial diversity?
PCR-based methods for rRNA gene analysis have been widely used to study diversity of microbiology. However, the analysis would be difficult when the DNA content in samples is too low to be amplified by convent...
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Article
Aspergillus niger lipase: gene cloning, over-expression in Escherichia coli and in vitro refolding
From the N-terminal amino acid sequence of the lipase from Aspergillus niger F044, a potential homologous gene A84689 to the lipanl (the gene encoding the lipase from Aspergillus niger F044) was identified. A pai...
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Article
Salicylic Acid Enhances Jaceosidin and Syringin Production in Cell Cultures of Saussurea medusa
Addition of 20 μM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l−1 and 631 mg l−1 which were, respectively, about ...
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Article
Repeated elicitation enhances taxane production in suspension cultures of Taxus chinensis in bioreactors
Methyl jasmonate added at 100 μM on days 8, 14 and 20 to cultures of Taxus chinensis growing in flasks and 1 l airlift bioreactors improved taxuyunnanine C (TC) production by 50–60% in flasks and by about 80% in ...
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Article
High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm
A cDNA of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator (mscu-PA) was constructed to include the natural scu-PA signal peptide sequences and transferred into the genome of Bombyx...
