10 Result(s)
within
Zhe** Yu
Applied Microbiology
Biochemistry, general
Biotechnology
Microbiology
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Article
A method to label biological molecules with dsDNA coated gold nanoparticles
We described a new method to label biological molecules using gold nanoparticles (GNPs) and double stranded DNA. Researchers can conveniently label their own samples with GNPs using this method. The label is b...
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Article
Source identification of bacterial and viral pathogens and their survival/fading in the process of wastewater treatment, reclamation, and environmental reuse
Pathogenic safety is drawing wide concern in water reclamation and reuse. In order to elucidate survive/fade of pathogens during the processes of wastewater treatment and reclamation, general indicators (fecal...
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Article
Analysis of the bacterial communities associated with different drinking water treatment processes
A drinking water plant was surveyed to determine the bacterial composition of different drinking water treatment processes (DWTP). Water samples were collected from different processing steps in the plant (i.e...
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Article
Isolation and characterization of an AHL lactonase gene from Bacillus amyloliquefaciens
An AHL lactonase gene (aiiA) was PCR amplified from the genomic DNA of Bacillus amyloliquefaciens, with the intact open reading frame of 753 base pair. The gene shares high identity to its homologues present in d...
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Article
Estimation of pullulan by hydrolysis with pullulanase
A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethyl...
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Article
Could nested PCR be applicable for the study of microbial diversity?
PCR-based methods for rRNA gene analysis have been widely used to study diversity of microbiology. However, the analysis would be difficult when the DNA content in samples is too low to be amplified by convent...
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Article
Aspergillus niger lipase: gene cloning, over-expression in Escherichia coli and in vitro refolding
From the N-terminal amino acid sequence of the lipase from Aspergillus niger F044, a potential homologous gene A84689 to the lipanl (the gene encoding the lipase from Aspergillus niger F044) was identified. A pai...
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Article
Salicylic Acid Enhances Jaceosidin and Syringin Production in Cell Cultures of Saussurea medusa
Addition of 20 μM salicylic acid to Saussurea medusa cell cultures at day 6 resulted in jaceosidin and syringin productions up to 95 mg l−1 and 631 mg l−1 which were, respectively, about ...
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Article
Repeated elicitation enhances taxane production in suspension cultures of Taxus chinensis in bioreactors
Methyl jasmonate added at 100 μM on days 8, 14 and 20 to cultures of Taxus chinensis growing in flasks and 1 l airlift bioreactors improved taxuyunnanine C (TC) production by 50–60% in flasks and by about 80% in ...
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Article
High level expression of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator in silkworm
A cDNA of a mutant (K151E, R154G) of single chain urokinase-type plasminogen activator (mscu-PA) was constructed to include the natural scu-PA signal peptide sequences and transferred into the genome of Bombyx...
