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Publisher Correction: Complementary Alu sequences mediate enhancer–promoter selectivity
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Complementary Alu sequences mediate enhancer–promoter selectivity
Enhancers determine spatiotemporal gene expression programs by engaging with long-range promoters1–4. However, it remains unknown how enhancers find their cognate promoters. We recently developed a RNA in situ co...
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Map** In Situ RNA–RNA Interactions with RIC-seq
Mammalian genomes encode large amounts of noncoding RNAs (ncRNAs), which tend to form intricate structures and interact with their target RNA molecules through complementary base pairing with the help of prote...
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Open AccessThe architecture of the SARS-CoV-2 RNA genome inside virion
SARS-CoV-2 carries the largest single-stranded RNA genome and is the causal pathogen of the ongoing COVID-19 pandemic. How the SARS-CoV-2 RNA genome is folded in the virion remains unknown. To fill the knowled...
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Global in situ profiling of RNA-RNA spatial interactions with RIC-seq
Emerging evidence has demonstrated that RNA-RNA interactions are vital in controlling diverse biological processes, including transcription, RNA splicing and protein translation. RNA in situ conformation seque...
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Global profiling of RNA-binding protein target sites by LACE-seq
RNA-binding proteins (RBPs) have essential functions during germline and early embryo development. However, current methods are unable to identify the in vivo targets of a RBP in these low-abundance cells. Her...
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RIC-seq for global in situ profiling of RNA–RNA spatial interactions
Highly structured RNA molecules usually interact with each other, and associate with various RNA-binding proteins, to regulate critical biological processes. However, RNA structures and interactions in intact ...
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Open AccessThe RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes
Activation-induced cytidine deaminase (AID) mediates class switching by binding to a small fraction of single-stranded DNA (ssDNA) to diversify the antibody repertoire. The precise mechanism for highly selecti...
