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Article
Genome editing reagent delivery in plants
Genome editing holds the potential for rapid crop improvement to meet the challenge of feeding the planet in a changing climate. The delivery of gene editing reagents into the plant cells has been dominated by...
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Protocol
Rice Gene Knockout or Downregulation through CRISPR-Cas9
Precise genome modification via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas)9 in rice plants, is a powerful tool for improving abiotic and biotic stress to...
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Article
Open AccessAn improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection
Cyst nematodes are biotrophs that form specialized feeding structures in the roots of host plants, which consist of a syncytial fusion of hypertrophied cells. The formation of syncytium is accompanied by profo...
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Protocol
Laser Microdissection of Plant Cells
Different plant cell types express unique transcriptomes, proteomes, and metabolomes. Therefore, the isolation of specific cell types prior to molecular analyses is important to understand the specification, d...
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Article
Atomic force microscopy visualises a hydrophobic meshwork in the central channel of the nuclear pore
Nuclear pore complexes (NPCs) mediate and control the transport of virtually all material between the cytosol and the nucleus. It is, therefore, unsurprising that they have long taken centre stage in physiolog...
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Protocol
Investigation of Nuclear Envelope Structure and Passive Permeability
We present an experimental approach by the help of which structure and passive permeability of the nuclear envelope (NE) can be investigated thoroughly, by combining imaging, fluorescent, and electrophysiologi...
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Article
Ethanol alters access to the cell nucleus
Ethanol is the most frequently used drug among humans. We tested the hypothesis whether ethanol, at clinically relevant concentrations modifies, signaling across the nuclear envelope (NE). In cell nuclei isola...
