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    Protocol

    Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos

    The two most common methods used to generate transgenic Xenopus embryos, restriction enzyme-mediated insertion, and I-SceI meganuclease take advantage of relatively common but spatially unpredictable double-stran...

    You E. Li, Bryan G. Allen, Daniel L. Weeks in Xenopus Protocols (2012)