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Article
Open AccessThe testis-specific E3 ubiquitin ligase RNF133 is required for fecundity in mice
Ubiquitination is a post-translational modification required for a number of physiological functions regulating protein homeostasis, such as protein degradation. The endoplasmic reticulum (ER) quality control ...
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Article
Open AccessSperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish
The process of sperm-egg fusion is critical for successful fertilization, yet the underlying mechanisms that regulate these steps have remained unclear in vertebrates. Here, we show that both mouse and zebrafi...
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Article
Open AccessForced expression of miR-143 and -145 in cardiomyocytes induces cardiomyopathy with a reductive redox shift
Animal model studies show that reductive stress is involved in cardiomyopathy and myopathy, but the exact physiological relevance remains unknown. In addition, the microRNAs miR-143 and miR-145 have been shown...
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Article
Open AccessLarge-scale discovery of male reproductive tract-specific genes through analysis of RNA-seq datasets
The development of a safe, effective, reversible, non-hormonal contraceptive method for men has been an ongoing effort for the past few decades. However, despite significant progress on elucidating the functio...
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Article
Open AccessIgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95
Osteoclasts are multinucleated, giant cells derived from myeloid progenitors. While receptor activator of NF-κB ligand (RANKL) stimulation is the primary driver of osteoclast differentiation, additional signal...
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Article
Open AccessJunB is essential for IL-23-dependent pathogenicity of Th17 cells
CD4+ T-helper cells producing interleukin-17 (IL-17), known as T-helper 17 (TH17) cells, comprise heterogeneous subsets that exhibit distinct pathogenicity. Although pathogenic and non-pathogenic TH17 subsets sha...
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Article
Open AccessCRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice
Targeted gene disrupted mice can be efficiently generated by expressing a single guide RNA (sgRNA)/CAS9 complex in the zygote. However, the limited success of complicated genome editing, such as large deletion...
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Article
Open AccessStructural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization
Sperm–egg fusion is the critical step in mammalian fertilization, and requires the interaction between IZUMO1 on the sperm surface and JUNO (also known as folate receptor (FR) 4 or IZUMO1R) on the egg surface....
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Article
Open AccessCalreticulin is required for development of the cumulus oocyte complex and female fertility
Calnexin (CANX) and calreticulin (CALR) chaperones mediate nascent glycoprotein folding in the endoplasmic reticulum. Here we report that these chaperones have distinct roles in male and female fertility. Canx nu...
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Article
Open AccessDouble strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes
The CRISPR/Cas system efficiently introduces double strand breaks (DSBs) at a genomic locus specified by a single guide RNA (sgRNA). The DSBs are subsequently repaired through non-homologous end joining (NHEJ)...
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Article
Open AccessFtx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos
X-chromosome inactivation (XCI) equalizes gene expression between the sexes by inactivating one of the two X chromosomes in female mammals. **st has been considered as a major cis-acting factor that inactivates t...
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Article
Open AccessGeneration of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA
CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide ...
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Article
Production of mouse pups from germline transmission-failed knockout chimeras
Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in ...
