-
Article
Open AccessGenome-wide analysis of the NAC gene family and functional verification of the DcNAC043s in Dendrobium catenatum
The NAC (NAM, ATAF1/2 and CUC2) domain protein plays an important role in plant growth and development. The function of members of NAC gene family has been deeply studied in many plants. However, the evolutionary...
-
Article
Open AccessMetagenomics-metabolomics analysis of microbial function and metabolism in petroleum-contaminated soil
Contamination of soil by petroleum is becoming increasingly serious in the world today. However, the research on gene functional characteristics, metabolites and distribution of microbial genomes in oil-conta...
-
Article
Cadmium-induced ultrastructural changes and apoptosis in the gill of freshwater mussel Anodonta woodiana
This study investigated the acute toxicity of cadmium (Cd) to the freshwater mussel Anodonta woodiana. The freshwater mussels were exposed to five concentrations of Cd (0 mg/L, 8.43 mg/L, 16.86 mg/L, 33.72 mg/L, ...
-
Article
A novel strategy of gene screen based on multi-omics in Streptomyces roseosporus
Daptomycin is a new lipopeptide antibiotic for treatment of severe infection caused by multi-drug-resistant bacteria, but its production cost remains high currently. Thus, it is very important to improve the f...
-
Article
Open AccessImprovement of FK506 production via metabolic engineering-guided combinational strategies in Streptomyces tsukubaensis
FK506, a macrolide mainly with immunosuppressive activity, can be produced by various Streptomyces strains. However, one of the major challenges in the fermentation of FK506 is its insufficient production, result...
-
Article
Activation and discovery of tsukubarubicin from Streptomyces tsukubaensis through overexpressing SARPs
Genome sequencing has revealed that each Streptomyces contains a wide range of biosynthetic gene clusters (BGCs) and has the capability to produce more novel natural products than what is expected. However, most ...
-
Article
Open AccessGenomic and transcriptomic survey of an endophytic fungus Calcarisporium arbuscula NRRL 3705 and potential overview of its secondary metabolites
Secondary metabolites as natural products from endophytic fungi are important sources of pharmaceuticals. However, there is currently little understanding of endophytic fungi at the omics levels about their po...
-
Article
Open AccessComprehensive dissection of dispensable genomic regions in Streptomyces based on comparative analysis approach
Large-scale genome reduction has been performed to significantly improve the performance of microbial chassis. Identification of the essential or dispensable genes is pivotal for genome reduction to avoid synt...
-
Article
Open AccessCrotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus
Due to the plethora natural products made by Streptomyces, the regulation of its metabolism are of great interest, whereas there is a lack of detailed understanding of the role of posttranslational modifications ...
-
Article
The regulatory cascades of antibiotic production in Streptomyces
Streptomyces is famous for its capability to produce the most abundant antibiotics in all kingdoms. All Streptomyces antibiotics are natural products, whose biosynthesis from the so-called gene clusters are elabo...
-
Article
Identification of a secondary metabolism-responsive promoter by proteomics for over-production of natamycin in Streptomyces
Streptomyces is currently the main producer of microbial pharmaceuticals from its secondary metabolites as natural products. It will be more beneficial if the promoters, which are particularly strong during the s...
-
Article
Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB
Genome sequencing projects revealed massive cryptic gene clusters encoding the undiscovered secondary metabolites in Streptomyces. To investigate the metabolic products of silent gene clusters in Streptomyces cha...
-
Article
Open AccessMolecular mechanism of azoxy bond formation for azoxymycins biosynthesis
Azoxy bond is an important chemical bond and plays a crucial role in high energy density materials. However, the biosynthetic mechanism of azoxy bond remains enigmatic. Here we report that the azoxy bond biosy...
-
Article
FadR1, a pathway-specific activator of fidaxomicin biosynthesis in Actinoplanes deccanensis Yp-1
Fidaxomicin, an 18-membered macrolide antibiotic, is highly active against Clostridium difficile, the most common cause of diarrhea in hospitalized patients. Though the biosynthetic mechanism of fidaxomicin has b...
-
Article
Dual regulation between the two-component system PhoRP and AdpA regulates antibiotic production in Streptomyces
Antibiotic production during secondary metabolism in Streptomyces spp. is elaborately controlled by multiple environmental signals and intracellular cascades. These include the two-component system PhoRP respondi...
-
Article
Open AccessRational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches
Streptomyces chattanoogensis L10 is the industrial producer of natamycin and has been proved a highly efficient host for diverse natural products. It has an enormous potential to be developed as a versatile cell ...
-
Article
Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces
Streptomyces are famed producers of secondary metabolites with diverse bioactivities and structures. However, biosynthesis of natural products will consume vast precursors from primary metabolism, and some second...
-
Article
Transposon-based identification of a negative regulator for the antibiotic hyper-production in Streptomyces
Production of secondary metabolites in Streptomyces is regulated by a complex regulatory network precisely, elaborately, and hierarchically. One of the main reasons for the low yields of some high-value secondary...
-
Article
Regulatory and biosynthetic effects of the bkd gene clusters on the production of daptomycin and its analogs A21978C1–3
Daptomycin is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus in an acidic peptide complex A21978C. In this complex, A21978C1–3 is most abundant and contains branched-chain fatty acyl groups,...
-
Article
Negative regulation of daptomycin production by DepR2, an ArsR-family transcriptional factor
Daptomycin, a lipopeptide antibiotic potently active against Gram-positive bacterial pathogens, is produced by Streptomyces roseosporus, but the transcriptional regulation on its biosynthesis is not fully underst...
