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Article
Open AccessCytokinetic engineering enhances the secretory production of recombinant human lysozyme in Komagataella phaffii
Human lysozyme (hLYZ) is a natural antibacterial protein with broad applications in food and pharmaceutical industries. Recombinant production of hLYZ in Komagataella phaffii (K. phaffii) has attracted considerab...
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Article
Open AccessPost-crotonylation oxidation by a monooxygenase promotes acetyl-CoA synthetase degradation in Streptomyces roseosporus
Protein post-translational modifications (PTMs) with various acyl groups play central roles in Streptomyces. But whether these acyl groups can be further modified, and the influences of these potential modificati...
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Article
Open AccessGenomic and transcriptomic survey of an endophytic fungus Calcarisporium arbuscula NRRL 3705 and potential overview of its secondary metabolites
Secondary metabolites as natural products from endophytic fungi are important sources of pharmaceuticals. However, there is currently little understanding of endophytic fungi at the omics levels about their po...
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Article
Open AccessComprehensive dissection of dispensable genomic regions in Streptomyces based on comparative analysis approach
Large-scale genome reduction has been performed to significantly improve the performance of microbial chassis. Identification of the essential or dispensable genes is pivotal for genome reduction to avoid synt...
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Article
Open AccessCrotonylation of key metabolic enzymes regulates carbon catabolite repression in Streptomyces roseosporus
Due to the plethora natural products made by Streptomyces, the regulation of its metabolism are of great interest, whereas there is a lack of detailed understanding of the role of posttranslational modifications ...
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Article
The regulatory cascades of antibiotic production in Streptomyces
Streptomyces is famous for its capability to produce the most abundant antibiotics in all kingdoms. All Streptomyces antibiotics are natural products, whose biosynthesis from the so-called gene clusters are elabo...
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Article
Identification of a secondary metabolism-responsive promoter by proteomics for over-production of natamycin in Streptomyces
Streptomyces is currently the main producer of microbial pharmaceuticals from its secondary metabolites as natural products. It will be more beneficial if the promoters, which are particularly strong during the s...
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Article
Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB
Genome sequencing projects revealed massive cryptic gene clusters encoding the undiscovered secondary metabolites in Streptomyces. To investigate the metabolic products of silent gene clusters in Streptomyces cha...
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Article
Open AccessMolecular mechanism of azoxy bond formation for azoxymycins biosynthesis
Azoxy bond is an important chemical bond and plays a crucial role in high energy density materials. However, the biosynthetic mechanism of azoxy bond remains enigmatic. Here we report that the azoxy bond biosy...
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Article
FadR1, a pathway-specific activator of fidaxomicin biosynthesis in Actinoplanes deccanensis Yp-1
Fidaxomicin, an 18-membered macrolide antibiotic, is highly active against Clostridium difficile, the most common cause of diarrhea in hospitalized patients. Though the biosynthetic mechanism of fidaxomicin has b...
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Article
Dual regulation between the two-component system PhoRP and AdpA regulates antibiotic production in Streptomyces
Antibiotic production during secondary metabolism in Streptomyces spp. is elaborately controlled by multiple environmental signals and intracellular cascades. These include the two-component system PhoRP respondi...
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Article
Open AccessRational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches
Streptomyces chattanoogensis L10 is the industrial producer of natamycin and has been proved a highly efficient host for diverse natural products. It has an enormous potential to be developed as a versatile cell ...
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Article
Transcriptome-Based Identification of a Strong Promoter for Hyper-production of Natamycin in Streptomyces
Streptomyces are famed producers of secondary metabolites with diverse bioactivities and structures. However, biosynthesis of natural products will consume vast precursors from primary metabolism, and some second...
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Article
Transposon-based identification of a negative regulator for the antibiotic hyper-production in Streptomyces
Production of secondary metabolites in Streptomyces is regulated by a complex regulatory network precisely, elaborately, and hierarchically. One of the main reasons for the low yields of some high-value secondary...
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Article
Regulatory and biosynthetic effects of the bkd gene clusters on the production of daptomycin and its analogs A21978C1–3
Daptomycin is a cyclic lipopeptide antibiotic produced by Streptomyces roseosporus in an acidic peptide complex A21978C. In this complex, A21978C1–3 is most abundant and contains branched-chain fatty acyl groups,...
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Article
Negative regulation of daptomycin production by DepR2, an ArsR-family transcriptional factor
Daptomycin, a lipopeptide antibiotic potently active against Gram-positive bacterial pathogens, is produced by Streptomyces roseosporus, but the transcriptional regulation on its biosynthesis is not fully underst...
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Article
Open AccessDevelopment of Series of Affinity Tags in Streptomyces
Streptomyces are of great biological and industrial significance due to their complex morphological development and ability to produce numerous secondary metabolites. However, the intrins...
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Article
Sigma factor WhiGch positively regulates natamycin production in Streptomyces chattanoogensis L10
The roles of many sigma factors are unclear in regulatory mechanism of secondary metabolism in Streptomyces. Here, we report the regulation network of a group 3 sigma factor, WhiGch, from a natamycin industrial s...
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Article
Construction of over-expression shuttle vectors in Streptomyces
Two high-copy number vectors, pL97 and pL98, that contain the strong constitutive ermEp* from Saccharopolyspora erythraea and the ssrA promoter (ssrAp) from Streptomyces coelicolor, respectively, and an inducible...
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Article
The ECF sigma factor SigT regulates actinorhodin production in response to nitrogen stress in Streptomyces coelicolor
Sigma factors of the extracytoplasmic function (ECF) subfamily are important regulators of stress responses in bacteria. This work described the characterization of ECF sigma factor SigT in Streptomyces coelicolo...
