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Article
Open AccessTranscriptomics profile of human bronchial epithelial cells exposed to ambient fine particles and influenza virus (H3N2)
Fine particulate matter (PM2.5) pollution remains a major threat to public health. As the physical barrier against inhaled air pollutants, airway epithelium is a primary target for PM2.5 and influenza viruses, tw...
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Article
Genomic analysis of a recombinant coxsackievirus A19 identified in **nxiang, China, in 2019
Coxsackievirus A19 (CV-A19) is an enterovirus belonging to the species Enterovirus C, and the prototype strain 8663 was isolated from a patient with Guillain-Barré syndrome in Japan. In this study, we determined ...
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Article
Short-term effect of NO2 on outpatient visits for dermatologic diseases in **nxiang, China: a time-series study
As the largest organ of the human body, the skin is the major exposure route of NO2. However, the evidence for a relationship between NO2 exposure and dermatologic diseases (DMs) is limited. This time-series stud...
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Article
Associations between air pollution and outpatient visits for allergic rhinitis in **nxiang, China
Several epidemiological studies have investigated the adverse health effects of air pollution, but studies reporting its effects on allergic rhinitis (AR) are limited, especially in develo** countries having...
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Article
A strip test for the optical determination of influenza virus H3 subtype using gold nanoparticle coated polystyrene latex microspheres
A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The Au...
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Article
Open AccessHapten Synthesis and the Development of an Ultrasensitive Indirect Competitive ELISA for the Determination of Diethylstilbestrol in Food Samples
An ultrasensitive indirect competitive enzyme-linked immunosorbent assay (ic ELISA) using monoclonal antibodies (mAbs) was developed for the specific detection of diethylstilbestrol (DES) residues. To establish a...
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Article
An Immunochromatographic Lateral Flow Strip Test for the Rapid Detection of Danofloxacin in Milk
An immunochromatographic lateral flow strip test (ILFST) for the rapid detection of danofloxacin (DAN) in milk samples was developed based on colloidal gold-labeled anti-DAN monoclonal antibody 3E9 and a compe...
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Article
Recombinant lactococcus lactis secreting viral protein 1 of enterovirus 71 and its immunogenicity in mice
To construct recombinant Lactococcus lactis (L. lactis) expressing viral protein 1 (VP1) of enterovirus 71 (EV71) and evaluate its immunogenicity to be used as an oral vaccine in BALB/c mice.
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Article
Open AccessA Lateral Flow Immunochromato-graphic Strip Test for Rapid Detection of Oseltamivir Phosphate in Egg and Chicken Meat
A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sens...
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Article
Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China
Porcine circovirus type 2 (PCV2) is an economically important pathogen in domestic pigs and wild boars all around the world. To understand the molecular epidemiology of PCV2 strains circulating in central Chin...
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Article
Open AccessMolecular epidemiology of hydropericardium syndrome outbreak-associated serotype 4 fowl adenovirus isolates in central China
In several parts of China, there have been a large number of hydropericardium syndrome (HPS) outbreaks caused by serotype 4 fowl adenovirus (FAdV4) in broiler chickens since 2015. These outbreak-associated FA...
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Article
Molecular epidemiology of outbreak-associated pseudorabies virus (PRV) strains in central China
In several parts of China, there have been a large number of pseudorabies (PR) outbreaks which have devastated many swine farms even though the herds had been previously immunized with gE-deleted vaccines (Bar...
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Article
Construction of a human scFv antibody library with VH regions randomized and its application
A non-immunized human single chain variable fragment (scFv) library containing 2.5 × 107 individual clones was constructed from antibody variable region genes of 200 non-immunized donors. ScFv gene repertories we...