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Recombinant lactococcus lactis secreting viral protein 1 of enterovirus 71 and its immunogenicity in mice

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Abstract

Objective

To construct recombinant Lactococcus lactis (L. lactis) expressing viral protein 1 (VP1) of enterovirus 71 (EV71) and evaluate its immunogenicity to be used as an oral vaccine in BALB/c mice.

Results

Recombinant L. lactis competent in secreting VP1 (~ 30 kDa) into the extracellular environment with the aid of the signal peptide Usp45 was produced. Enzyme-linked immunosorbent assay showed that significant VP1-specific antibody response including the production of both serum IgG and fecal IgA (p < 0.05) was elicited in BALB/c mice upon oral immunization with recombinant L. lactis. Moreover, in contrast to negative control, recombinant L. lactis induced adequate neutralizing antibodies in mouse sera (p < 0.05) as demonstrated in virus neutralization assay, whereas the presence of neutralizing antibodies in fecal samples was obvious but not significant (p > 0.05).

Conclusions

Recombinant L. lactis expressing VP1 of EV71 has the potential to be used as an oral vaccine candidate. The findings may provide some preliminary evidences for further development of effective and needle-free EV71 vaccines.

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Acknowledgements

This work was supported by the Key Scientific Research Project of Universities of Henan Province, China (Grant Nos. 19A330004 and 18B360012).

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Author notes

  1. Pengwei Xu and Yinbiao Wang have contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, **nxiang Medical University, **nxiang, 453003, China

    Pengwei Xu, Yinbiao Wang, Ling Tao & Weidong Wu

  2. School of Life Science, Sichuan University, Chengdu, 610064, China

    Xuan Wu

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  1. Pengwei Xu
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  2. Yinbiao Wang
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  3. Ling Tao
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  4. Xuan Wu
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  5. Weidong Wu
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Correspondence to Pengwei Xu.

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Xu, P., Wang, Y., Tao, L. et al. Recombinant lactococcus lactis secreting viral protein 1 of enterovirus 71 and its immunogenicity in mice. Biotechnol Lett 41, 867–872 (2019). https://doi.org/10.1007/s10529-019-02695-1

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  • DOI: https://doi.org/10.1007/s10529-019-02695-1

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