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Immunoprophylaxis and Immunotherapy of Respiratory Syncytial Virus–Infected Mice with Respiratory Syncytial Virus–Specific Immune Serum
ABSTRACT: The effects of passive respiratory syncytial virus (RSV) immune serum given as preinoculation prophylaxis, postinoculation prophylaxis, and as therapy on d 5 after inoculation were evaluated in an il...
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Protocol
A Colorimetric Microtiter Plate Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2
Herpes simplex virus (HSV) is an ubiquitous agent responsible for a wide variety of human infections. In addition to epithelial infections such as gingivostomatitis, pharyngitis, genital herpes, whitlow, conju...
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Book
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Chapter
In Vitro Nucleic Acid Amplification: An Introduction
Over the past decade, the development of a series of in vitro nucleic acid ampli- fication (NAA) technologies has opened new avenues for the detection, identifi- cation, and characterization of pathogenic organis...
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Chapter
Amplification Product Inactivation
The extreme sensitivity of PCR has proved to be one of the strong points of this laboratory technique, making it possible to detect even small numbers of infectious agents present in a clinical sample. It is u...
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Protocol
Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens
Rapid and accurate detection of respiratory syncytial virus (RSV) provides pathogen-specific diagnosis, allows implementation of appropriate infection control measures, and improves patient management. One dia...
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Article
Molecular Diagnosis of Viral Respiratory Infections
In clinical practice, a rapid and accurate identification of pathogens causing viral respiratory tract infections can be problematic because of nonspecific clinical presentations, lack of rapid and sensitive t...
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Article
Covalent imprinted polymer for selective and rapid enrichment of ractopamine by a noncovalent approach
A novel molecularly imprinted polymer (MIP) for the separation and concentration of ractopamine (RAC) was prepared by a covalent imprinting approach and the template was removed successfully by hydrolysis, so ...
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Article
Methicillin-resistant Staphylococcus aureus bloodstream infections in a Japanese University Hospital between 1987 and 2004
Methicillin-resistant Staphylococcus aureus (MRSA) infections have been the most common cause of nosocomial infections in Japan, but their genetic characteristics related to bloodstream infections have not been w...
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Article
Open AccessImmune Mediators of protective and pathogenic immune responses in patients with mild and fatal human monocytotropic ehrlichiosis
Ehrlichia chaffeens is is a bacterial pathogen that causes fatal human monocytic ehrlichiosis (HME) that mimic toxic shock-like syndrome. Murine studies indicate that over activation of cellular immunity followed...
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Book
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Chapter
Amplification Product Inactivation
“With great power comes great responsibility.” This popular Spider Man movie quote infers the relationship between molecular nucleic acid amplification methods and amplification product inactivation procedures...
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Chapter
An Introduction to In Vitro Nucleic Acid Amplification Techniques
A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and...
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Chapter
An Introduction to Amplification–Production–Detection Techniques
This decade has seen the use of molecular detection techniques grow in popularity within the field of diagnostic microbiology. The importance of molecular methods in the detection of infectious disease agents ...
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Chapter
Diagnosis and Assessment of Microbial Infections with Host and Microbial microRNA Profiles
Biomarkers are continuously being sought in the field of diagnostic microbiology for the laboratory diagnosis and assessment of microbial infections. A set of clinical and laboratory criteria necessary for an ...
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Chapter
Interpretation and Relevance of Advanced Technique Results
Advanced techniques in the field of diagnostic microbiology have made amazing progress over the past two decades due largely to a technological revolution in the molecular aspects of microbiology [1, 2]. In pa...
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Article
Respiratory virus multiplex RT-PCR assay sensitivities and influence factors in hospitalized children with lower respiratory tract infections
Multiplex RT-PCR assays have been widely used tools for detection and differentiation of a panel of respiratory viral pathogens. In this study, we evaluated the Qiagen ResPlex II V2.0 kit and explored factors ...
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Article
Nasal vestibulitis due to targeted therapies in cancer patients
Cancer patients treated with targeted therapies (e.g., epidermal growth factor receptor inhibitors) are susceptible to dermatologic adverse events (AEs) including secondary skin infections. Whereas infections ...
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Article
Laboratory Diagnosis of Central Nervous System Infection
Central nervous system (CNS) infections are potentially life threatening if not diagnosed and treated early. The initial clinical presentations of many CNS infections are non-specific, making a definitive etio...
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Article
Detection of mycobacterial and viral DNA in Kikuchi-Fujimoto disease: an analysis of 153 Chinese pediatric cases
