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Open AccessReal-time, random-access organ screening for carbapenem-resistant organisms (CRO) reduces CRO-associated, donor-derived infection mortality in lung transplant recipients
Donor-derived infection (DDI) has become an important factor affecting the prognosis of lung transplantation patients. The risks versus benefits of using donor organs infected with multidrug-resistant organism...
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Article
Open AccessA comprehensive update on CIDO: the community-based coronavirus infectious disease ontology
The current COVID-19 pandemic and the previous SARS/MERS outbreaks of 2003 and 2012 have resulted in a series of major global public health crises. We argue that in the interest of develo** effective and saf...
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Chapter
Diagnostic Tests and Procedures During the COVID-19 Pandemic
Coronavirus disease 2019 (COVID-19) has brought a huge impact on global health and the economy. Early and accurate diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections is essent...
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Article
Open AccessCase control study comparing the HPV genome in patients with oral cavity squamous cell carcinoma to normal patients using metagenomic shotgun sequencing
The aim of this study was to carry out a case control study comparing the HPV genome in patients with oral cavity squamous cell carcinoma (OC-SCC) to normal patients using metagenomic shotgun sequencing. We re...
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Chapter
Molecular Diagnostics in Central Nervous System Infections
Central nervous system (CNS) infections can be life threatening if not diagnosed and treated early. The myriad clinical presentations of CNS infections can make a prompt diagnosis challenging. Multiple diagnos...
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Article
Open AccessEffects of parental genetic divergence on gene expression patterns in interspecific hybrids of Camellia
The merging of two divergent genomes during hybridization can result in the remodeling of parental gene expression in hybrids. A molecular basis underling expression change in hybrid is regulatory divergence, ...
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Chapter
Impacts and Challenges of Advanced Diagnostic Assays for Transplant Infectious Diseases
The advanced technologies described in this chapter should allow for full inventories to be made of bacterial genes, their time- and place-dependent expression, and the resulting proteins as well as their outc...
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Article
Fecal calprotectin concentrations in cancer patients with Clostridium difficile infection
Fecal calprotectin (fCPT) has been used as a surrogate marker for assessment of intestinal inflammation. We explore the utility of fCPT values as a diagnostic aid in cancer patients with suspected Clostridium dif...
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Article
Simultaneous detection and characterization of toxigenic Clostridium difficile directly from clinical stool specimens
We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC fo...
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Chapter
Advanced Methods for Screening and Identification of Methicillin-Resistant Staphylococcus aureus
Methicillin-resistant Staphylococcus aureus (MRSA) is among the most important causes of human infections in the twenty-first century and is found in both hospitals and communities. Early and accurate detection a...
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Chapter
Interpretation and Relevance of Advanced Technique Results
Advanced techniques in the field of diagnostic microbiology have made amazing progress over the past 25 years due largely to a technological revolution in the molecular aspects of microbiology [1, 2]. In parti...
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Chapter
Laboratory Diagnosis of HIV-1 Infections: State of the Art
Significant technical advances have been made in the detection and characterization of human immunodeficiency virus (HIV) infections since this virus was determined to be the pathogen causing AIDS. These techn...
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Article
Detection of mycobacterial and viral DNA in Kikuchi-Fujimoto disease: an analysis of 153 Chinese pediatric cases
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Article
Laboratory Diagnosis of Central Nervous System Infection
Central nervous system (CNS) infections are potentially life threatening if not diagnosed and treated early. The initial clinical presentations of many CNS infections are non-specific, making a definitive etio...
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Article
Nasal vestibulitis due to targeted therapies in cancer patients
Cancer patients treated with targeted therapies (e.g., epidermal growth factor receptor inhibitors) are susceptible to dermatologic adverse events (AEs) including secondary skin infections. Whereas infections ...
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Article
Respiratory virus multiplex RT-PCR assay sensitivities and influence factors in hospitalized children with lower respiratory tract infections
Multiplex RT-PCR assays have been widely used tools for detection and differentiation of a panel of respiratory viral pathogens. In this study, we evaluated the Qiagen ResPlex II V2.0 kit and explored factors ...
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Book
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Chapter
Amplification Product Inactivation
“With great power comes great responsibility.” This popular Spider Man movie quote infers the relationship between molecular nucleic acid amplification methods and amplification product inactivation procedures...
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Chapter
An Introduction to In Vitro Nucleic Acid Amplification Techniques
A molecular microbiology detection procedure consists of three basic components: (1) nucleic acid extraction, (2) amplification, and (3) detection/identification. Over the past two decades, the development and...
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Chapter
An Introduction to Amplification–Production–Detection Techniques
This decade has seen the use of molecular detection techniques grow in popularity within the field of diagnostic microbiology. The importance of molecular methods in the detection of infectious disease agents ...
