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Article
Enhancing (2S)-naringenin production in Saccharomyces cerevisiae by high-throughput screening method based on ARTP mutagenesis
(2S)-Naringenin, a dihydro-flavonoid, serves as a crucial precursor for flavonoid synthesis due to its extensive medicinal values and physiological functions. A pathway for the synthesis of (2S)-naringenin from g...
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Article
Engineering Saccharomyces cerevisiae for synthesis of β-myrcene and (E)-β-ocimene
Monoterpenes are among the important natural plant terpenes. Monoterpenes usually have the characteristics of volatility and strong aroma. β-Myrcene and its isomer (E)-β-ocimene are typical acyclic monoterpenes. ...
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Article
Sedimentary Records of the Dramatic Environmental Changes in the Lingdingyang Bay of the Pearl River Estuary in Southern China
To understand the spatial distribution and vertical evolution of sediments in the Pearl River Estuary in recent decades, two sediment cores (80 cm long) from the western and eastern areas of the outer Lingding...
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Article
Open AccessEfficient 1,3-dihydroxyacetone biosynthesis in Gluconobacter oxydans using metabolic engineering and a fed-batch strategy
1,3-Dihydroxyacetone (DHA) is a commercially important chemical and widely used in cosmetics, pharmaceuticals, and food industries as it prevents excessive water evaporation, and provides anti-ultraviolet radi...
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Article
Open AccessProduction of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source
Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production....
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Article
Optimization of citrinin production by endophytic Penicillium citrinum isolated from Ziziphus jujuba
Citrinin is a common mycotoxin produced by different fungi. Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs, few methods are...
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Article
Comparing measurement correction of echo sounder in shallow-water area
Error correction of echo sounder is very important for the accurate measurement of water-depth in shallow-water area. There are some issues in measuring the water depth, they are accuracy, resolution, inaccura...
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Article
Open AccessO-Acetyl-L-homoserine production enhanced by pathway strengthening and acetate supplementation in Corynebacterium glutamicum
O-Acetyl-L-homoserine (OAH) is an important potential platform chemical. However, low levels of production of OAH are greatly limiting its industrial application. Furthermore, as a common and safe amino acid-p...
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Article
Transcriptome Analysis of Gluconobacter oxydans WSH-003 Exposed to Elevated 2-Keto-L-Gulonic Acid Reveals the Responses to Osmotic and Oxidative Stress
Industrial production of 2-keto-L-gulonic acid (2-KLG), the precursor of vitamin C, is mainly achieved by a two-step fermentation process carried out by Gluconobacter oxydans, Bacillus, and Ketogulonicigenium. On...
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Article
Open AccessActive tyrosine phenol-lyase aggregates induced by terminally attached functional peptides in Escherichia coli
The formation of inclusion bodies (IBs) without enzyme activity in bacterial research is generally undesirable. Researchers have attempted to recovery the enzyme activities of IBs, which are commonly known as ...
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Article
Open AccessObtaining a series of native gradient promoter-5′-UTR sequences in Corynebacterium glutamicum ATCC 13032
Corynebacterium glutamicum is an important industrial microorganism used for the production of many valuable compounds, especially amino acids and their derivatives. For fine-tuning of metabolic pathways, synthet...
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Article
Integrating enzyme evolution and high-throughput screening for efficient biosynthesis of l-DOPA
l-DOPA is a key pharmaceutical agent for treating Parkinson’s, and market demand has exploded due to the aging population. There are several challenges associated with the chemical synthesis of l-DOPA, including ...
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Article
Separation and purification of α-ketoglutarate and pyruvate from the fermentation broth of Yarrowia lipolytica
A strategy to achieve the efficient co-production of α-ketoglutarate (KGA) and pyruvate (PYR) via Yarrowia lipolytica fermentation was established in our previous work. The next big challenge is to achieve an eff...
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Article
Enhanced avermectin production by Streptomyces avermitilis ATCC 31267 using high-throughput screening aided by fluorescence-activated cell sorting
Avermectins, produced by Streptomyces avermitilis, are important antiparasitic agents. The use of traditional microbial breeding methods for this organism has been limited by the low-throughput shake flask-based ...
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Article
A high-throughput screening procedure for enhancing pyruvate production in Candida glabrata by random mutagenesis
Candida glabrata is one of the most promising pyruvate producers. A series of experiments was conducted to enhance pyruvate production by C. glabrata via metabolic and genetic engineering...
