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Article
The modification of heme special importer to improve the production of active hemoglobins in Escherichia coli
To enhance the import of heme for the production of active hemoproteins in Escherichia coli C41 (DE3) lacking the special heme import system, heme receptor ChuA from E. coli Nissle 1917 was modified through molec...
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Article
Construction of short synthetic promoters for optimization of ovalbumin expression level in Saccharomyces cerevisiae
Saccharomyces cerevisiae, a common eukaryotic model microorganism that is generally recognized as safe, is widely employed for the heterologous expression of edible and therapeutic proteins. Promoters are crucial...
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Article
Construction of a plasmid-free Escherichia coli strain for lacto-N-neotetraose biosynthesis
Lacto-N-neotetraose (LNnT), with promising bioactive properties, is one of the most significant nonfucosylated human milk saccharides. Recently, its synthesis by microbial fermentation has attracted great attenti...
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Article
Construction of immobilized enzyme cascades for the biosynthesis of nucleotide sugars UDP-N-acetylglucosamine and UDP-glucuronic acid
UDP-sugars, as active forms of monosaccharides, play integral roles in glycosylation and biosynthesis of polysaccharides. Although enzymatic catalysis has achieved great process, the comparatively low producti...
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Article
Metabolic engineering of Bacillus subtilis for de novo synthesis of 6′-sialyllactose
6′-sialyllactose (6′-SL) is an important component of human milk oligosaccharides (HMOs) and has numerous infant health benefits. The construction of efficient and food-safe microbial cell factories to produce 6′
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Article
Optimization of linear plasmid expression system for protein production and secretion in Bacillus thuringiensis
Bacillus thuringiensis, a safe bacterium widely used in agriculture for the biocontrol of pests, has great potential for protein production. The linear plasmid expression system, bacterial orthogonal DNA replicat...
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Article
Open AccessUltrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering
Classical directed evolution is a powerful approach for engineering biomolecules with improved or novel functions. However, it traditionally relies on labour- and time-intensive iterative cycles, due in part t...
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Article
Engineered Saccharomyces cerevisiae for de novo δ-tocotrienol biosynthesis
As a vitamin E isomer, δ-tocotrienol has attracted much attention owing to its rich biological activities for human health, especially anticancer activities. Microbial biosynthesis constitutes a promising stra...
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Article
Engineering Bacillus subtilis for efficient production of 3′-sialyllactose
3′-Sialyllactose (3′-SL) promotes the proliferation of the bifidobacterial population and shapes gut microbiota. Thus, it can be used in the infant formula industry. In this study, an engineered Bacillus subtilis
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Article
Engineered bacterial orthogonal DNA replication system for continuous evolution
Continuous evolution can generate biomolecules for synthetic biology and enable evolutionary investigation. The orthogonal DNA replication system (OrthoRep) in yeast can efficiently mutate long DNA fragments i...
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Article
Open AccessHigh-throughput droplet microfluidics screening and genome sequencing analysis for improved amylase-producing Aspergillus oryzae
The exceptional protein secretion capacity, intricate post-translational modification processes, and inherent safety features of A. oryzae make it a promising expression system. However, heterologous protein expr...
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Article
Open AccessEngineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds
Sulfonation as one of the most important modification reactions in nature is essential for many biological macromolecules to function. Development of green sulfonate group donor regeneration systems to efficie...
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Article
Open AccessAnalysis of acid-tolerance mechanism based on membrane microdomains in Saccharomyces cerevisiae
Saccharomyces cerevisiae has been used in the biosynthesis of acid products such as organic acids owing to its acid tolerance. Improving the acid tolerance of S. cerevisiae is beneficial for expanding its applica...
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Article
Crude enzyme immobilization-based cell-free system for efficient N-acetylneuraminic acid biosynthesis aided by N-terminal coding sequence screening
N-acetylneuraminic acid (NeuAc) is an important nutrient that plays a key role in brain development in infants NeuAc is mainly produced by extraction from natural resources such as edible birds’s nests, crucian.....
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Article
Combinatorial metabolic engineering enables high yield production of α-arbutin from sucrose by biocatalysis
α-Arbutin has been widely used as a skin-whitening ingredient. Previously, we successfully produced α-arbutin via whole-cell biocatalysis and found that the conversion rate of sucrose to α-arbutin was low (~45...
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Article
Enhanced catalytic efficiency and substrate specificity of Streptomyces griseus trypsin by evolution-guided mutagenesis
Streptomyces griseus trypsin (SGT) is a bacteria-sourced trypsin that could be potentially applied to industrial insulin productions. However, SGT produced by microbial hosts displayed low catalytic efficiency an...
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Article
In silico cell factory design driven by comprehensive genome-scale metabolic models: development and challenges
Genome-scale metabolic models (GEMs) have been widely used to design cell factories in silico. However, initial flux balance analysis only considers stoichiometry and reaction direction constraints, so it cann...
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Article
CRISPR–dCas12a-mediated genetic circuit cascades for multiplexed pathway optimization
The production efficiency of microbial cell factories is sometimes limited by the lack of effective methods to regulate multiple targets in a coordinated manner. Here taking the biosynthesis of glucosamine-6-p...
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Article
Revealing novel synergistic defense and acid tolerant performance of Escherichia coli in response to organic acid stimulation
Escherichia coli is an important producer of mono- and di-acids, such as D-lactic acid, itaconic acid, and succinic acid. However, E. coli has limited acid tolerance and requires neutralizers in large-scale ferme...
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Article
Open AccessEngineering sigma factors and chaperones for enhanced heterologous lipoxygenase production in Escherichia coli
Lipoxygenase (EC. 1.13.11.12, LOX) can catalyze the addition of oxygen into polyunsaturated fatty acids to produce hydroperoxides, which are widely used in the food, chemical, and pharmaceutical industries. In...
