33 Result(s)

Article

Author Correction: Advances and prospects for the Human BioMolecular Atlas Program (HuBMAP)

Sanjay Jain, Liming Pei, Jeffrey M. Spraggins, Michael Angelo… in Nature Cell Biology (2024)

Article

2H-Thiopyran-2-thione sulfine, a compound for converting H₂S to HSOH/H₂S₂ and increasing intracellular sulfane sulfur levels

Reactive sulfane sulfur species such as persulfides (RSSH) and H₂S₂ are important redox regulators and closely linked to H₂S signaling. However, the study of these species is still challenging due to their instab...

Qi Cui, Meg Shieh, Tony W. Pan, Akiyuki Nishimura… in Nature Communications (2024)

Article

The mitochondrially targeted peptide elamipretide (SS-31) improves ADP sensitivity in aged mitochondria by increasing uptake through the adenine nucleotide translocator (ANT)

Aging muscle experiences functional decline in part mediated by impaired mitochondrial ADP sensitivity. Elamipretide (ELAM) rapidly improves physiological and mitochondrial function in aging and binds directly...

Gavin Pharaoh, Varun Kamat, Sricharan Kannan, Rudolph S. Stuppard… in GeroScience (2023)

Article

Harnessing redox proteomics to study metabolic regulation and stress response in lignin-fed Rhodococci

Rhodococci are studied for their bacterial ligninolytic capabilities and proclivity to accumulate lipids. Lignin utilization is a resource intensive process requiring a variety of redox active enzymes and cofacto...

**aolu Li, Austin Gluth, Song Feng… in Biotechnology for Biofuels and Bioproducts (2023)

Article

Elamipretide effects on the skeletal muscle phosphoproteome in aged female mice

The age-related decline in skeletal muscle mass and function is known as sarcopenia. Sarcopenia progresses based on complex processes involving protein dynamics, cell signaling, oxidative stress, and repair. W...

Matthew D. Campbell, Miguel Martín-Pérez, Jarrett D. Egertson… in GeroScience (2022)

Article

Exploratory study reveals far reaching systemic and cellular effects of verapamil treatment in subjects with type 1 diabetes

Currently, no oral medications are available for type 1 diabetes (T1D). While our recent randomized placebo-controlled T1D trial revealed that oral verapamil had short-term beneficial effects, their duration a...

Guanlan Xu, Tiffany D. Grimes, Truman B. Grayson, Junqin Chen… in Nature Communications (2022)

Article

Tutorial: best practices and considerations for mass-spectrometry-based protein biomarker discovery and validation

Mass-spectrometry-based proteomic analysis is a powerful approach for discovering new disease biomarkers. However, certain critical steps of study design such as cohort selection, evaluation of statistical pow...

Ernesto S. Nakayasu, Marina Gritsenko, Paul D. Piehowski, Yuqian Gao… in Nature Protocols (2021)

Article

Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics

Large numbers of cells are generally required for quantitative global proteome profiling due to surface adsorption losses associated with sample processing. Such bulk measurement obscures important cell-to-cel...

Chia-Feng Tsai, Pengfei Zhang, David Scholten, Kendall Martin… in Communications Biology (2021)

Article

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440

Efficient utilization of all available carbons from lignocellulosic biomass is critical for economic efficiency of a bioconversion process to produce renewable bioproducts. However, the metabolic responses tha...

Zhangyang Xu, Chunmei Pan, **aolu Li, Naijia Hao, Tong Zhang… in Biotechnology for Biofuels (2021)

Article

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains

Biological routes for utilizing both carbohydrates and lignin are important to reach the ultimate goal of bioconversion of full carbon in biomass into biofuels and biochemicals. Recent biotechnology advances h...

**aolu Li, Yucai He, Libing Zhang, Zhangyang Xu, Haoxi Ben… in Biotechnology for Biofuels (2019)

Article

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of Comamonas serinivorans SP-35

The efficient depolymerization and utilization of lignin are one of the most important goals for the renewable use of lignocelluloses. The degradation and complete mineralization of lignin by bacteria represen...

Daochen Zhu, Haibing Si, Peipei Zhang, Alei Geng… in Biotechnology for Biofuels (2018)

Article

Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells

There is an unmet technical challenge for mass spectrometry (MS)-based proteomic analysis of single mammalian cells. Quantitative proteomic analysis of single cells has been previously achieved by antibody-bas...

Tu** Shi, Matthew J. Gaffrey, Thomas L. Fillmore… in Communications Biology (2018)

Article

Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells

Nanoscale or single-cell technologies are critical for biomedical applications. However, current mass spectrometry (MS)-based proteomic approaches require samples comprising a minimum of thousands of cells to ...

Ying Zhu, Paul D. Piehowski, Rui Zhao, **g Chen, Yufeng Shen… in Nature Communications (2018)