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Article
Successful application of modified crude DNA extraction from muscle tissues for various types of PCR amplifications
One of the most challenging aspects of nucleic acid amplification tests is the extraction of genomic DNA. However, achieving satisfactory quality and quantity of genomic DNA is not always easy, while the deman...
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Article
Development and validation of a universal primer pair for the taxonomic and phylogenetic studies of vertebrates
Recent studies in the field of molecular identification have described 16S rRNA gene as a highly informative fragment of mitochondrial DNA for species discrimination. This study presents a newly developed univ...
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Article
Description of novel variants in consanguineous Pakistani families affected with intellectual disability
Intellectual disability (ID) is a neurodevelopmental condition, affecting 1–3% of the population. Genetic factors play a key role causing the limitation in intellectual functioning and adaptive behavior. The h...
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Article
Vitamin E boosted the protective potential of Aloe vera in CCl4-treated rats
Aloe vera is a medicinal plant widely used for biomedical, pharmaceutical, and cosmetic applications. Oodles studies are going on to evaluate the protective role of Aloe vera while treating it along with differen...
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Article
Open AccessSequencing the CaSR locus in Pakistani stone formers reveals a novel loss-of-function variant atypically associated with nephrolithiasis
Nephrolithiasis (NL) affects 1 in 11 individuals worldwide and causes significant morbidity and cost. Common variants in the calcium sensing receptor gene (CaSR) have been associated with NL. Rare inactivating Ca...
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Article
Whole exome sequencing revealed novel variants in consanguineous Pakistani families with intellectual disability
Intellectual disability (ID) is a heterogeneous disorder affecting 1–3% of the population. Elucidation of monogenic variants for ID is a current challenge. These variants can be better demonstrated in consangu...
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Article
Assessment of nematodes in Punjab Urial (Ovis vignei punjabiensis) population in Kalabagh Game Reserve: development of a DNA barcode approach
Punjab urial (Ovis vignei punjabiensis) is a wild sheep of Pakistan, considered a vulnerable species by IUCN. Major threats to urial populations include habitat loss and poaching, causing severe declines in its p...
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Article
Open AccessLow genetic diversity of Ehrlichia canis associated with high co-infection rates in Rhipicephalus sanguineus (s.l.)
Rhipicephalus sanguineus sensu lato (s.l.) is the most widely distributed ixodid tick and is a vector of major canine and human pathogens. High-throughput technologies have revealed that individual ticks carry a ...
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Article
Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)
Given the global evolutionary dynamics of Newcastle disease viruses (NDVs), it is imperative to continue extensive surveillance, routine monitoring and characterization of isolates originating from natural res...
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Article
Development and validation of a loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis in mastitic milk
Mycoplasma mastitis is often difficult to control due to a lack of rapid and accurate diagnostic tools. The aim of the current study was to develop a loop-mediated isothermal amplification (LAMP)...
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Article
Molecular characterization of Hepatozoon canis from farm dogs in Pakistan
Hepatozoon canis is a tick-borne pathogen of canids, which is distributed worldwide. However, very little is known about this protozoan parasite in Pakistan. This study provides the first molecular evidence of H....
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Article
Induction of specific humoral immune response in mice immunized with ROP18 nanospheres from Toxoplasma gondii
Toxoplasmosis is one of the most common zoonotic protozoal diseases. Recent advances in biotechnology have produced recombinant protein, which are immunogenic, and progress in nano-pharmaceutics has generated ...
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Article
Open AccessAn In silico approach for the evaluation of DNA barcodes
DNA barcoding is a key tool for assessing biodiversity in both taxonomic and environmental studies. Essential features of barcodes include their applicability to a wide spectrum of taxa and their ability to id...
