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Article
Open AccessImpact of Limosilactobacillus fermentum probiotic treatment on gut microbiota composition in sahiwal calves with rotavirus diarrhea: A 16S metagenomic analysis study”
Diarrhea poses a major threat to bovine calves leading to mortality and economic losses. Among the causes of calf diarrhea, bovine rotavirus is a major etiological agent and may result in dysbiosis of gut micr...
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Article
Open AccessInvestigation of milk microbiota of healthy and mastitic Sahiwal cattle
Sahiwal cattle is an indigenous cattle breed of Pakistan and mastitis is one of the major problems faced by Sahiwal cattle which hinders its production potential. The study was designed to investigate the milk...
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Article
Temporal study of wastewater surveillance from September 2020 to March 2021: an estimation of COVID-19 patients in Lahore, Pakistan
The first aim of study was to quantify the viral load in the wastewater samples by RT-qPCR testing in Lahore population to estimate the number of patients affected and predict the next resurgence of COVID-19 w...
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Article
Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care Platform
Development of lab-on-a-chip (LOC) system based on integration of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and microfluidic technology is expected to speed up SARS-CoV-2 diagnosti...
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Article
An in vitro antiviral activity of iodine complexes against SARS-CoV-2
Since the emergence of COVID-19 pandemic in China in late 2019, scientists are striving hard to explore non-toxic, viable anti-SARS-CoV-2 compounds or medicines. We determined In vitro anti-SARS-CoV-2 activity...
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Article
Sero-Epidemiological Survey of Crimean-Congo Hemorrhagic Fever among the Human Population of the Punjab Province in Pakistan
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Article
Circulation of multiple subtypes (A, G and CRFs 02_AG) of human immunodeficiency virus type 1 (HIV-1) in selected districts of Punjab province, Pakistan
Owing to consistent genetic mutation and recombination, various escape mutants and/or drug-resistant mutants of human immunodeficiency virus (HIV-1) are now emerging worldwide. Therefore, an understanding of t...
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Article
The first report on seroty** of bluetongue virus in small ruminants of Khyber Pakhtunkhwa province, Pakistan
Bluetongue virus (BTV), a member of Orbivirus genus (family Reoviridae), is a non-contagious infection of domestic and wild ruminants. The current study was designed to detect various serotypes of BTV in small ru...
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Article
Genomic and biological characterization of Newcastle disease viruses isolated from migratory mallards (Anas platyrhynchos)
Given the global evolutionary dynamics of Newcastle disease viruses (NDVs), it is imperative to continue extensive surveillance, routine monitoring and characterization of isolates originating from natural res...
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Article
Development and validation of a loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis in mastitic milk
Mycoplasma mastitis is often difficult to control due to a lack of rapid and accurate diagnostic tools. The aim of the current study was to develop a loop-mediated isothermal amplification (LAMP)...
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Article
Open AccessAn international collaborative study to determine the prevalence of contagious caprine pleuropneumonia by monoclonal antibody-based cELISA
Few serological tests are available for detecting antibodies against Mycoplasma capricolum subsp. capripneumoniae, the causal agent of contagious caprine pleuropneumonia (CCPP). The complement fixation test, the ...
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Article
Open AccessMolecular epidemiology and viral load of HCV in different regions of Punjab, Pakistan
Hepatitis C virus (HCV) is highly infectious pathogen which is responsible for causing Hepatitis around 200 million individuals worldwide. In Pakistan, 4.7% of HCV prevalence has been reported and HCV genotype...
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Article
Open AccessInfectivity and transmissibility of H9N2 avian influenza virus in chickens and wild terrestrial birds
Genetic changes in avian influenza viruses influence their infectivity, virulence and transmission. Recently we identified a novel genotype of H9N2 viruses in widespread circulation in poultry in Pakistan that...
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Article
Open AccessGenetic diversity of Newcastle disease virus in Pakistan: a countrywide perspective
Newcastle disease (ND) is one of the most deadly diseases of poultry around the globe. The disease is endemic in Pakistan and recurrent outbreaks are being reported regularly in wild captive, rural and commerc...
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Article
Genetic analysis of Newcastle disease virus from Punjab, Pakistan
The strains of Newcastle disease virus (NDV) were isolated from five suspected outbreaks of ND in broiler (n = 3) and layers (n = 2) poultry farms. The egg-isolated viruses were subjected to biological and geneti...
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Article
Open AccessPrevalence of HCV genotypes in district Mardan
Approximately 170 million people are infected with Hepatitis C virus (HCV) worldwide. The prevalence of chronic HCV infections in Pakistan is about 5%, with most individuals being infected with HCV genotype 3a...
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Chapter and Conference Paper
Parametric Representation of the Environment of a Mobile Robot for Measurement Update in a Particle Filter
A mobile robot must know its position and heading, all the time during navigation. This is called localization. Recently particle filters [1] have become extremely popular for position estimation. These are si...
