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Article
A novel method for monitoring protoplast fusion
Two fluorescent compounds, scopoletin and carboxyfluorescein, have been used to label both tissue culture and leaf mesophyll cells and protoplasts. The compounds localized within the vacuoles of cells in appro...
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Article
Distribution of golgi apparatus-associated polyribosomes across the polarity axis of dictyosomes of wild carrot (Daucus carota L.)
Endoplasmic reticulum-polyribosome-Golgi apparatus associations were a general feature of cells of suspension cultures of wild carrot (Daucus carota L.). Free polyribosomes occurred within the Golgi apparatus zon...
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Article
Fractionation of suspension cultures of wild carrot and kinetics of membrane labeling
Wild carrot (Daucus carota L.) cells, grown in suspension culture, were labeled with radioactive precursors and fractionated into constituent membranes to be analyzed for specific radioactivity. Results show rapi...
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Article
Calcium-induced fusion of fusogenic wild carrot protoplasts
Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the re...
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Chapter
Developmentally Regulated Fusion of Carrot Protoplasts
Protoplast fusion can be induced by PEG, high calcium at high pH, nitrate, and electric field stimulus. However, with all of these treatments, increasing the percentage of fusion beyond a certain threshold dec...
