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  1. No Access

    Chapter and Conference Paper

    A 38 kDa Protein Resident to CIS Golgi Apparatus Cisternae of Rat Liver is Recognized by an Antibody Directed Against α Subunits of Trimeric G-Proteins

    Using a completely cell-free system from rat liver, we have demonstrated the formation of 50–70 nm ATP-dependent transfer vesicles and the transfer of both lipids and proteins to Golgi apparatus immobilized on...

    A. O. Brightman, M. Paulik, J. B. Lawrence… in Molecular Mechanisms of Membrane Traffic (1993)

  2. No Access

    Article

    Identification of tonoplast fractions resolved from plasma membrane by free-flow electrophoresis using filipin-labeling and antibody to the tonoplast ATPase

    Preparative free-flow electrophoresis has been employed in combination with density gradient centrifugation to prepare fractions enriched in either tonoplast or plasma membrane from dark grown seedlings of cre...

    B. vom Dorp, G. F. E. Scherer, H. Canut, A. O. Brightman, Christa Liedtke… in Protoplasma (1990)

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    Article

    Transitional endoplasmic reticulum membranes and vesicles isolated from animals and plants

    The process of formation from endoplasmic reticulum and transfer to Golgi apparatus of small 50–70 nm transition vesicles has been reconstituted in a cell-free system. Fractions enriched in transition elements...

    D. J. Morré, D. D. Nowack, M. Paulik, A. O. Brightman, Karin Thornborough… in Protoplasma (1989)

  4. No Access

    Article

    A novel method for monitoring protoplast fusion

    Two fluorescent compounds, scopoletin and carboxyfluorescein, have been used to label both tissue culture and leaf mesophyll cells and protoplasts. The compounds localized within the vacuoles of cells in appro...

    K. Kanchanapoom, A. O. Brightman, H. D. Grimes, W. F. Boss in Protoplasma (1985)