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Article
High amylopectin in waxy maize synergistically affects seed germination and seedling vigour over traditional maize genotypes
Waxy maize grains rich in amylopectin have emerged as a popular food and industrial raw materials. Here, a set of waxy inbreds having recessive waxy1 (wx1) gene derived through marker-assisted selection (MAS), an...
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Article
Analysis of nutritional composition in opaque2- and crtRB1-based single- and double-biofortified super sweet corn
Sweet corn has emerged as a favorite food item worldwide owing to its kernel sweetness. However, traditional sweet corn cultivars are poor in provitamin-A (proA) and essential amino acids, viz., lysine and try...
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Article
Unique genetic architecture of prolificacy in ‘Sikkim Primitive’ maize unraveled through whole-genome resequencing-based DNA polymorphism
‘Sikkim Primitive’ maize landrace, unique for prolificacy (7–9 ears per plant) possesses unique genomic architecture in branching and inflorescence-related gene(s), and locus Zm00001eb365210 encoding glycosyltran...
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Article
Allelic variation and haplotype diversity of Matrilineal (MTL) gene governing in vivo maternal haploid induction in maize
Diverse haploid inducer lines with > 6% of haploid induction rate are now routinely used to develop doubled haploid lines. Though MTL gene regulates haploid induction, its molecular characterization and haplotype...
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Article
Open AccessPrediction of matrilineal specific patatin-like protein governing in-vivo maternal haploid induction in maize using support vector machine and di-peptide composition
The mutant matrilineal (mtl) gene encoding patatin-like phospholipase activity is involved in in-vivo maternal haploid induction in maize. Doubling of chromosomes in haploids by colchicine treatment leads to comp...
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Article
Assessing sequence variation, haplotype analysis and molecular characterisation of aspartate kinase2 (ask2) gene regulating methionine biosynthesis in diverse maize inbreds
Traditional maize grain is deficient in methionine, an essential amino acid required for proper growth and development in humans and poultry birds. Thus, development of high methionine maize (HMM) assumes grea...
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Article
Low expression of lipoxygenase 3 (LOX3) enhances the retention of kernel tocopherols in maize during storage
Deficiency of vitamin E results in several neurological and age-related disorders in humans. Utilization of maize mutants with favourable vte4-allele led to the development of several α-tocopherol (vitamin E) ric...
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Article
Enhancement of nutritional quality in maize kernel through marker-assisted breeding for vte4, crtRB1, and opaque2 genes
Traditional maize is poor in vitamin-E [α-tocopherol (α-T): 6–8 ppm], vitamin-A [provitamin-A (proA): 1–2ppm], lysine (0.150–0.2–50%), and tryptophan (0.030–0.040%). Here, we combined favourable alleles of vte4,
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Article
Transgenerational effects of thermal stress on reproductive physiology of fall armyworm, Spodoptera frugiperda
Temperature is one of the critical abiotic factors influencing the range, richness, activity, life history and viability of insects. The persistence trend of thermal stress on biological and reproductive trait...
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Article
Development of novel gene-based markers for waxy1 gene and their validation for exploitation in molecular breeding for enhancement of amylopectin in maize
Waxy corn possessing high amylopectin is widely employed as an industrial product. Traditional corn contains ~ 70–75% amylopectin, whereas waxy corn with the mutant waxy1 (wx1) gene possesses ~ 95–100% amylopecti...
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Article
Development of Ripening Gene-Specific Markers and their Association with Shelf-Life in Mango (Mangifera indica L.) varieties
Majority of the popular mango varieties have a very short shelf-life. Therefore, the present investigation was carried out to design functional markers and their association with shelf-life. A total of 35 shel...
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Article
Provitamin A, lysine and tryptophan enrichment in shrunken2-based sweet corn genotypes through genomics-assisted breeding for crtRB1 and opaque2 genes
Malnutrition affects large section of population worldwide. Vitamin A and protein deficiencies have emerged as the major global health-issue. Traditional shrunken2 (sh2)-based sweet corn is deficient in provitami...
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Article
Variation in anthocyanin pigmentation by R1-navajo gene, development and validation of breeder-friendly markers specific to C1-Inhibitor locus for in-vivo haploid production in maize
In-vivo maternal haploids serve as the basis of doubled haploid (DH) breeding in maize. R1-navajo (R1-nj) gene governing anthocyanin colouration in the endosperm and embryo is widely used to identify haploid seed...
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Chapter
Genetic Improvement of Specialty Corn for Nutritional Quality Traits
Specialty corn has emerged as a preferred choice as food, feed, and various industrial products due to its diverse usage. Among the various types, sweet corn, popcorn, waxy corn, high amylose corn, high oil co...
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Chapter
Dissection of QTLs for Biotic Stress Resistance in Maize
Biotic stresses are major concerns for the world’s food security. These, commonly induced by diseases and/or insect pests, are one of the primary reasons for maize yield and quality losses. About 10% of the an...
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Article
Correction to: Pollen staining is a rapid and cost‑effective alternative to marker‑assisted selection for recessive waxy1 gene governing high amylopectin in maize
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Article
Characterization of crtRB1- and vte4-based biofortified sweet corn inbreds for seed vigour and physico-biochemical traits
Sweet corn possessing recessive shrunken2 (sh2) gene is popular worldwide. Traditional sweet corn is poor in vitamin A and vitamin E. Plant breeders during the selection of sweet corn genotypes mainly emphasize o...
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Article
Pollen staining is a rapid and cost-effective alternative to marker-assisted selection for recessive waxy1 gene governing high amylopectin in maize
Waxy maize is popular for food-, feed- and industrial usage. It possesses a recessive waxy1 (wx1) gene that enhances amylopectin to ~ 95–100%, compared to ~ 70–75% in traditional maize. Marker-assisted selection ...
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Article
Open AccessDevelopment of β-carotene, lysine, and tryptophan-rich maize (Zea mays) inbreds through marker-assisted gene pyramiding
Maize (Zea mays L.) is the leading cereal crop and staple food in many parts of the world. This study aims to develop nutrient-rich maize genotypes by incorporating crtRB1 and o2 genes associated with increased β...
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Article
Development and validation of rapid and cost-effective protocol for estimation of amylose and amylopectin in maize kernels
Maize possesses wide variation in amylose and amylopectin which assumes significance as a part of both food-chain and different industrial applications. Estimation of amylose and amylopectin in maize kernels i...
