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    Protocol

    In Vitro Induction of Teleost PGCs

    are unique cells in an . These cells contain all genetic information and therefore represent the best source to store maternal and paternal genomes until is achieved. However, the number of these cells i...

    Vanesa Robles, David G. Valcarce, Marta F. Riesco in Germline Development in the Zebrafish (2021)

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    Protocol

    Chapter 19 Cryopreservation Effect on Genetic Function: Neonatal Outcomes

    Cryopreservation is a well-established technique commonly used in clinical practice. It is used widely for the conservation of gametes and embryos that will be used later for insemination or in vitro fertiliza...

    Vanesa Robles, Marta F. Riesco in Cryopreservation of Mammalian Gametes and … (2017)

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    Protocol

    Flow Cytometric Methods for Sperm Assessment

    Flow cytometry allows the assessment of multiple sperm parameters following a diverse number of protocols. Here, we describe three methods for evaluating critical aspects of sperm quality: the double stain SYB...

    Vanesa Robles, Felipe Martínez-Pastor in Spermatogenesis (2013)